CAG-GCaMP3 AAV (Serotype 9)

Name: CAG-GCaMP3 AAV (Serotype 9)
SKU: AAB0019
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAB0019

Serotype : AAV Serotype 9 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAB0019
Description	Premade AAV particles in serotype 9 containing GCaMP3 under the control of a CAG promoter.
Product Type	Adeno-associated virus particles
Tag	GCaMP3
Serotype	AAV Serotype 9
Biosensor	GCaMP3-Green calcium indicator
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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The discovery that AAV9 can cross the blood-brain barrier (BBB) was the starting point for extensive investigation of its therapeutic effects in a variety of central nervous system (CNS) diseases, of which intravenous administration is the main route of administration. In general, AAV9 is used to repair defective genes, as in the case of lysosomal storage diseases and spinal muscular atrophy type 1; to silence dominantly inherited mutant alleles, as in Huntington's disease; or to introduce disease-modifying genes that may alleviate neuropathology, as in the study of Alzheimer's disease.

Theoretically, given the natural properties of vascular AAV9-mediated gene therapy, the most suitable candidates for vascular AAV9-mediated gene therapy are diseases that: i) have a clear genetic cause, so the affected gene can be directly targeted; ii) present with early onset, which is conducive to vector administration in childhood, when neuronal tropism may be stronger; iii) affect both the CNS and peripheral organs, which is compatible with the natural AAV9 biodistribution.

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Customer Reviews

Outstanding Customer Support

In addition to the remarkable qualities of the CAG-GCaMP3 AAV (Serotype 9), the customer support provided by the company has exceeded our expectations. They are responsive, knowledgeable, and eager to assist with any inquiries.

Canada

2022-10-05

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CAG-GCaMP3 AAV (Serotype 9)

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Outstanding Customer Support

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