Cat.No. : AAB0023
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 9 Storage: -80 ℃
| Cat. No. | AAB0023 |
| Description | Premade AAV particles in serotype 9 containing Cre-dependent GCaMP6m under the control of a CAG promoter. |
| Serotype | AAV Serotype 9 |
| Tag | GCaMP6m |
| Product Type | Adeno-associated virus particles |
| Biosensor | GCaMP6m-Improved SNR, intermediate kinetics; Green indicator |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Social behavior is essential for human connection and belonging, and often influences disorders such as autism spectrum disorder (ASD). The mesoaccumbens pathway (VTA and NAc) plays a key role in social behavior and has been implicated in ASD. Here, researchers used the Shank3-deficient rat model to examine the effects of Shank3 mutations on the midbrain nucleus accumbens pathway during behavior. The results showed that Shank3-deficient rats displayed atypical social interactions and had difficulty adjusting behavior based on reward value, which was associated with altered neuronal activity in VTA dopaminergic and GABAergic neurons and reduced dopamine release in the NAc. Furthermore, the study demonstrated that manipulation of VTA neuronal activity normalized this behavior, providing insight into the effects of Shank3 mutations on social reward and behavior and identifying potential neural pathways for intervention.
To investigate the effects of Shank3 mutations on the neural activity of VTA-GABA neurons during social interactions, the researchers injected the VTA with AAV9-CAG-FLEX-GCaMP6m virus, which is responsible for expressing GCaMP6 in a Cre-dependent manner, and rAAV-hVGAT1-Cre-WPRE-hGH polyA virus, which expresses Cre recombinase under the control of the vesicular GABA transporter (vGAT) promoter, which is commonly used to target GABAergic neurons60 (Figures 1A and 1B). Recordings showed that while WT rats showed a slight increase in VTA-GABA activity when approaching social stimuli, both Shank3-Het and Shank3-KO rats showed significantly higher increases in VTA-GABA activity (Figures 1C-E). In the social vs. food task (Figure 1F), the researchers observed a similar trend of increased VTA-GABA activity only when rats approached social stimuli but not food stimuli (Figure 1G-I and Figure 1J-L, respectively). Taken together, these findings suggest that Shank3-deficient rats exhibit abnormal increases in VTA-GABA neural activity that are specific to social interactions and not universal to all stimuli.
Figure 1. Shank3-deficient rats show abnormal increase in VTA-GABA neural activity during social interaction. (Barbier M, et al., 2023)
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I’m impressed by the neuronal specificity of the Syn-FLEX-NES-jRCaMP1a AAV (Serotype 8). It precisely targets our cell types of interest, reducing off-target effects and improving the quality of our experiments.
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