Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : AAV00208Z
Serotype : AAV Serotype 6 Storage : -80 ℃
Titer: Size:
| Cat. No. | AAV00208Z |
| Description | AAV serotype 6 particles contain FLPo recombinase under the Synapsin promoter. |
| Serotype | AAV Serotype 6 |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Different AAV serotypes display a range of properties related to antigenicity, in vivo tropism, and receptor interactions based on their capsid structure. The capsids of different AAV strains bind to a range of cell surface glycan receptors and utilize coreceptors for infection. These differences in capsid-receptor interactions play an important role in determining the regional and cellular transduction efficiency of AAV strains in different mammalian organs.
Over the past two decades, advances in the understanding of the biology of AAV infection have provided the scientific and clinical communities with a range of AAV strains with desirable features for CNS gene transfer. In addition to natural isolates, several laboratory-derived AAV strains have been engineered or evolved for specific CNS gene transfer applications. These efforts have resulted in novel AAV vectors for targeting (a) glioblastoma cells; (b) neural stem cells in rats, mice, and humans; and (c) specific regions (piriform cortex and ventral hippocampus) in rats with compromised blood-brain barrier (BBB).
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The AAV6-Syn-FLPo's ability to efficiently deliver genes specifically to neuronal cells makes it invaluable for studying complex neural networks.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.