Cat.No. : AAV00314Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV serotype PHP.S Storage: -80 ℃
| Cat. No. | AAV00314Z |
| Description | AAV serotype PHP.S particles contain GFP under human Synapsin promoter. |
| Reporter | GFP |
| Serotype | AAV serotype PHP.S |
| Application | 1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery. |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
Motor recovery after severe spinal cord injury (SCI) is limited due to the disruption of direct descending commands. Despite the absence of descending input from the brain, sensory afferents below the injury site remain intact. Among them, proprioception is an important sensory source that regulates local spinal circuits and determines motor output. Here, researchers established a viral system to selectively target lumbar proprioceptive neurons, and then introduced excitatory Gq-coupled designer drug-exclusively activated receptors (DREADD) viruses into proprioceptors to achieve specific activation of lumbar proprioceptive neurons after CNO administration. The study showed that chronic activation of lumbar proprioceptive neurons promoted the recovery of hindlimb stepping ability in a bilateral hemisection SCI mouse model. Further studies found that chemical genetic proprioceptive stimulation can coordinately activate proprioceptive spinal interneurons and promote the transmission of supraspinal commands to lumbar motor neurons without affecting the regeneration of proprioceptive afferents or brain-derived descending axons. These findings suggest that proprioception-based combinatorial modalities may be a promising strategy to restore motor function after severe spinal cord injury.
Here, researchers injected AAV/PHP.S-Syn-GFP (AAV/PHP.S-GFP) intrathecally into PV-Cre::Rosa26-tdTomato (RTM) reporter mice (PV-RTM) (Figure 1A). Two weeks later, approximately 80.95% of PV+ proprioceptors in lumbar DRGs were found to be labeled by AAV/PHP.S-GFP virus, while only 22.30% were labeled in cervical DRGs (Figures 1B and 1C). In contrast, despite the high density of PV+ interneurons in the brain, almost no neurons were labeled in the cerebral cortex and cerebellum (Figure 1E). In the superficial dorsal horn of the spinal cord, some neurons that did not overlap with PV+ interneurons were labeled by GFP (Figure 1D). Together, these data indicate that intrathecal injection of PHP.S virus can effectively and preferentially label lumbar DRGs.
Figure 1. Intrathecal injection of AAV/PHP.S preferentially and efficiently targets lumbar DRGs. (Gao Z, et al., 2021)
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Creative Biogene's AAV PHP.S-Syn-GFP product provided reliable and consistent results. Their customer service was also very helpful, answering all our technical questions promptly.
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