Panoply™ Human TNFRSF1A Over-expressing Stable Cell Line

Name: Panoply™ Human TNFRSF1A Over-expressing Stable Cell Line
SKU: CSC-SC016363
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016363

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC016363
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFRSF1A
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ]
Gene Symbol	TNFRSF1A
Synonyms	TNFRSF1A; tumor necrosis factor receptor superfamily, member 1A; TNFR1; tumor necrosis factor receptor superfamily member 1A; CD120a; TNF R; TNF R I; TNF R55; TNFAR; TNFR60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta; FPF; p55; p60; TBP1; TNF-R; p55-R; TNFR55; TNF-R-I; TNF-R55; MGC19588;
GeneID	7132
Uni ProtID	P19438
mRNA Refseq	BC010140
Chromosome Location	12p13.2
Function	protease binding; protein binding; receptor activity
Pathway	Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem;
MIM	191190

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Oxygen-glucose deprivation/reoxygenation (OGD/R)-mediated renal ischemia frequently results in increased apoptosis and heightened inflammation. Sap, a flavonoid extracted from Caesalpinia sappan L., possesses various cytoprotective activities. Here, researchers investigated the effects of Sap on HK-2 cells under OGD/R treatment. The results showed that Sap may be associated with renal ischemia. Furthermore, Sap alleviated OGD/R-mediated HK-2 cell damage by increasing cell viability and inhibiting apoptosis and inflammation. Sap also inhibited the activation of the TNFRSF1A/NF-κB signaling pathway. Moreover, upregulation of TNFRSF1A attenuated the inhibitory effect of Sap on OGD/R-mediated apoptosis and inflammation. In conclusion, Sap alleviates OGD/R-induced HK-2 cell damage by downregulating the TNFRSF1A/NF-κB signaling pathway, thus providing a theoretical basis for the treatment of renal ischemia.

To investigate whether TNFRSF1A overexpression could reverse the inhibitory effect of SAP in HK-2 cells, researchers conducted a rescue experiment. The results showed that in TNFRSF1A-overexpressing HK-2 cells treated with OGD/R and SAP, increased apoptosis rate (Figure 1A), enhanced LDH release (Figure 1B), and increased caspase-3 activity (Figure 1C) reversed the effects of SAP. Furthermore, the results confirmed that, compared to the OGD/R+Sap group, the expression levels of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-18 were significantly increased in TNFRSF1A-overexpressing HK-2 cells (Figures 2A-D). These results demonstrate that SAP alleviated HK-2 cell damage in the OGD/R model in a dose-dependent manner by downregulating TNFRSF1A.

Figure 1. TNFRSF1A overexpression mitigated the suppressive influence of Sap on OGD/R-mediated cell death. (Wang Q, et al., 2022)

Figure 2. TNFRSF1A upregulation lessened the suppressive effect of Sap on OGD/R-mediated inflammation. (Wang Q, et al., 2022)

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