Panoply™ Human TNFRSF11B Over-expressing Stable Cell Line

Name: Panoply™ Human TNFRSF11B Over-expressing Stable Cell Line
SKU: CSC-SC016355
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016355

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC016355
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TNFRSF11B
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
Gene Symbol	TNFRSF11B
Synonyms	OPG; TR1; OCIF
Gene Description	tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Gene ID	4982
Uni Prot ID	O00300
m RNA Refseq	NM_002546.3
Protein Refseq	NP_002537.3
Chromosome Location	8q24
Function	cytokine activity; receptor activity;
Pathway	Apoptosis Modulation and Signaling, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Monoamine Transport, organism-specific biosystem; Osteoblast Signaling, organism-specific biosystem; Osteoclast Signaling, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem;
MIM	602643

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TNFRSF11B is involved in the development and progression of various malignancies. However, its function in bladder cancer and its associated carcinogenic mechanisms are less well understood. Here, researchers evaluated the clinical significance of TNFRSF11B in bladder cancer and its associated signaling pathways through bioinformatics analysis. The expression levels of TNFRSF11B were measured in bladder tissue and bladder cancer cells. To investigate the effect of the PI3K/AKT pathway on TNFRSF11B, experiments were performed using the PI3K/AKT inhibitor LY294002. The results showed that TNFRSF11B was significantly upregulated in bladder cancer tissue and various bladder cancer cell lines. Inhibition of TNFRSF11B expression inhibited the proliferation, migration, and invasion of bladder cancer cells. Overexpression of TNFRSF11B, however, had the opposite effect. These findings were further confirmed by in vivo experiments. Furthermore, TNFRSF11B promotes the malignant progression of bladder cancer by regulating the PI3K/AKT pathway. In summary, TNFRSF11B is highly expressed in bladder cancer and plays an important role in promoting cancer through the PI3K/AKT pathway. Therefore, TNFRSF11B may become a potential prognostic marker for bladder cancer treatment.

To validate the mechanism by which TNFRSF11B promotes tumorigenesis through the PI3K/AKT pathway, researchers conducted rescue experiments to confirm its pro-proliferative effect on bladder cancer cells. TNFRSF11B-overexpressing cells were treated with the PI3K/AKT inhibitor LY294002. CCK-8 and LDH assays showed that LY294002 significantly reduced the cell viability of TNFRSF11B-overexpressing T24 and 5637 cells (Figure 1A and B). Transwell invasion and migration assays demonstrated that LY294002 significantly inhibited the invasion and migration of TNFRSF11B-overexpressing T24 and 5637 cells (Figure 1C and D). Wound healing assay further confirmed that LY294002 inhibited the migration of bladder cancer cells (Figure 1E). Furthermore, researchers investigated whether TNFRSF11B exerts its oncogenic effects by inhibiting apoptosis. Flow cytometry results showed that LY294002 reversed the inhibitory effect of TNFRSF11B overexpression on cell apoptosis. Therefore, TNFRSF11B promotes tumor progression by inhibiting apoptosis in bladder cancer cells.

Figure 1. LY294002 inhibits the cancer-promoting activity of TNFRSF11B via the PI3K/AKT pathway. (Deng H, et al., 2024)

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