Cat.No. : CSC-SC015292 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC015292 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | STEAP1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | STEAP1 six transmembrane epithelial antigen of the prostate 1 [ Homo sapiens ] |
| Gene Symbol | STEAP1 |
| Synonyms | STEAP; PRSS24 |
| Gene Description | six transmembrane epithelial antigen of the prostate 1 |
| Gene ID | 26872 |
| UniProt ID | Q9UHE8 |
| mRNA Refseq | NM_012449.2 |
| Protein Refseq | NP_036581.1 |
| Chromosome Location | 7q21 |
| Function | channel activity; metal ion binding; oxidoreductase activity; transporter activity; |
| Pathway | Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; |
| MIM | 604415 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The six-transmembrane epithelial antigen of the prostate 1 (STEAP1) gene is associated with the development and progression of cancer. Here, researchers aimed to elucidate the role of STEAP1 in gastric cancer tumor growth and metastasis and its molecular mechanisms. The results showed that STEAP1 is overexpressed in gastric cancer tissues and cell lines. Univariate and Cox regression analyses showed that STEAP1 gene expression levels were associated with poor prognosis. Upregulation of STEAP1 increased cell proliferation, migration, and invasion, while knockdown of STEAP1 reduced these changes. These changes were mediated by activation of the AKT/FoxO1 pathway and epithelial-mesenchymal transition (EMT). In vivo animal studies demonstrated that knockdown of STEAP1 reduced subcutaneous and peritoneal tumor formation.
CCK-8 assay results showed that in SGC-7901 and MGC-803 cell lines, the absorbance in the STEAP1 knockdown group was lower than that in the control group at 48, 72, and 96 hours (Figure 1A). In STEAP1-overexpressing SGC-7901 and MGC-803 cell lines, the absorbance was higher at 24, 48, 72, and 96 hours than in the control group (Figure 1B). CCK-8 assay results suggest that STEAP1 can affect cell proliferation. In colony formation assays, the number of colonies in the STEAP1 knockdown group was lower than that in the control group (Figure 1C), whereas the number of colonies in the STEAP1 overexpression group was higher than that in the empty vector group (Figure 1D). The results of colony formation assays also demonstrated that STEAP1 can affect cell proliferation. Next, flow cytometry was used to assess the cell cycle. Results showed that in STEAP1 knockdown SGC-7901 and MGC-803 cells, the proportion of cells in the S phase decreased, while the proportions of cells in the G0/G1 and G2/M phases increased (Figure 1E). In SGC-7901 and MGC-803 cells overexpressing STEAP1, the proportion of cells in the S phase increased, while the proportions of cells in the G0/G1 and G2/M phases decreased (Figure 1F). Flow cytometry analysis indicated that the STEAP1 gene may affect cell proliferation by influencing the cell cycle.
Figure 1. Functional experiments related to cell proliferation in the SGC-7901 and MGC-803 cell lines. (Zhang Z, et al., 2020)
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