Cat.No. : CSC-SC008717 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC008717 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | LIMK1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | Limk1 LIM-domain containing, protein kinase [ Mus musculus ] |
| Gene Symbol | Limk1 |
| Gene Description | LIM-domain containing, protein kinase |
| Gene ID | 16885 |
| UniProt ID | P53668 |
| mRNA Refseq | NM_010717.2 |
| Protein Refseq | NP_034847.1 |
| Chromosome Location | 5 G2; 5 75.0 cM |
| Function | ATP binding; heat shock protein binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups; zinc ion binding; |
| Pathway | Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; G13 Signaling Pathway, organism-specific biosystem; |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
LIMK1 is a serine protease that is highly expressed in various tumors and is closely associated with tumor initiation, progression, and metastasis. Here, researchers investigated the mechanisms of LIMK1's role in cervical cancer progression. The study showed that LIMK1 overexpression promoted tumor growth in nude mice. Cell scratch assays, Transwell assays, and colony formation assays demonstrated that LIMK1 promoted the invasion, migration, and proliferation of cervical cancer cells. Western blotting results showed that LIMK1 promoted the expression of ROS-related proteins NOX2, NOX4, p-Src, and their downstream proteins p-FAK, p-ROCK1/2, p-Cofilin-1, and F-actin, while inhibiting the expression of p-SHP2 protein. Correction experiments indicated that LIMK1 regulates the expression of p-FAK and p-Cofilin-1 proteins by regulating ROS and p-Src. By examining the function of cervical cancer cells, it was found that LIMK1-induced ROS and p-Src activation are early events that promote cervical cancer cell migration, proliferation, and invasion.
To investigate the effect of LIMK1 on the proliferative capacity of cervical cancer cells, researchers conducted a colony formation assay. The results showed that the number of colonies in LIMK1-overexpressing (LIMK1-OE) SiHa and CaSki cells was significantly higher than that of the control group (NC group). Compared with the control group, the number of colonies in LIMK1-knockdown (LIMK1-KD) SiHa and CaSki cells was significantly reduced (Figure 1).
Figure 1. LIMK1 promoted the proliferation of cervical cancer cells. (Jia Y, et al., 2024)
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