Panoply™ Human LIMK1 Over-expressing Stable Cell Line

Name: Panoply™ Human LIMK1 Over-expressing Stable Cell Line
SKU: CSC-SC008717
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC008717

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC008717
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	LIMK1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LIMK1 LIM domain kinase 1 [ Homo sapiens ]
Gene Symbol	LIMK1
Synonyms	LIMK; LIMK-1
GeneID	3984
Uni ProtID	P53667
mRNA Refseq	NM_001204426.1
Protein Refseq	NP_001191355.1
Chromosome Location	7q11.23
Function	ATP binding; heat shock protein binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threonine kinase activity; zinc ion binding;
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem;
MIM	601329

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LIMK1 is a serine protease that is highly expressed in various tumors and is closely associated with tumor initiation, progression, and metastasis. Here, researchers investigated the mechanisms of LIMK1's role in cervical cancer progression. The study showed that LIMK1 overexpression promoted tumor growth in nude mice. Cell scratch assays, Transwell assays, and colony formation assays demonstrated that LIMK1 promoted the invasion, migration, and proliferation of cervical cancer cells. Western blotting results showed that LIMK1 promoted the expression of ROS-related proteins NOX2, NOX4, p-Src, and their downstream proteins p-FAK, p-ROCK1/2, p-Cofilin-1, and F-actin, while inhibiting the expression of p-SHP2 protein. Correction experiments indicated that LIMK1 regulates the expression of p-FAK and p-Cofilin-1 proteins by regulating ROS and p-Src. By examining the function of cervical cancer cells, it was found that LIMK1-induced ROS and p-Src activation are early events that promote cervical cancer cell migration, proliferation, and invasion.

To investigate the effect of LIMK1 on the proliferative capacity of cervical cancer cells, researchers conducted a colony formation assay. The results showed that the number of colonies in LIMK1-overexpressing (LIMK1-OE) SiHa and CaSki cells was significantly higher than that of the control group (NC group). Compared with the control group, the number of colonies in LIMK1-knockdown (LIMK1-KD) SiHa and CaSki cells was significantly reduced (Figure 1).

Figure 1. LIMK1 promoted the proliferation of cervical cancer cells. (Jia Y, et al., 2024)

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