Panoply™ Human KLRK1 Over-expressing Stable Cell Line

Name: Panoply™ Human KLRK1 Over-expressing Stable Cell Line
SKU: CSC-SC008330
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC008330

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC008330
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	KLRK1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
Gene Symbol	KLRK1
Synonyms	KLR; CD314; NKG2D; NKG2-D; D12S2489E
GeneID	22914
Uni ProtID	P26718
mRNA Refseq	NM_007360.3
Protein Refseq	NP_031386.2
Chromosome Location	12p13.2-p12.3
Pathway	Adaptive Immune System, organism-specific biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Malaria, organism-specific biosystem;
MIM	611817

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Lung cancer is one of the most common malignancies worldwide, resulting in approximately 1.6 million deaths annually. Cancer immune surveillance has been found to play a significant role in lung cancer and may be associated with its prognosis. KLRK1, encoding NKG2D, is a homodimeric lectin-like receptor. However, studies investigating KLRK1 as a lung cancer biomarker are limited. Here, researchers downloaded data from the TCGA database, including patient clinical characteristics and KLRK1 RNAseq information. A total of 1,019 lung cancer patients (407 female and 611 male) were included. The study demonstrated that KLRK1 expression is lower in tumors than in normal tissues. KLRK1 expression is associated with sex, histological grade, stage, T stage, and vital status. Patients with high KLRK1 expression have prolonged overall and recurrence-free survival. Analysis of overall survival in the subgroups of lung adenocarcinoma, stage I/II, elderly patients, and male patients, as well as recurrence-free survival in the subgroups of lung adenocarcinoma, stage I/II, elderly patients, and male patients, revealed significant prognostic value for KLRK1. Lung adenocarcinoma patients with high KLRK1 expression have improved overall and recurrence-free survival. In vitro studies have shown that KLRK1 can inhibit tumor cell proliferation and migration. KLRK1 is an independent prognostic factor, and high KLRK1 expression is associated with improved overall and recurrence-free survival. KLRK1 may serve as a prognostic biomarker for lung adenocarcinoma.

The researchers overexpressed KLRK1 in lung cancer A549 cells to investigate its effects on cells. CCK-8 assays showed that cell proliferation was significantly inhibited in KLRK1-overexpressing cells (Figure 1A). Compared with the control group, the percentage of dead cells (live/dead staining) in KLRK1-overexpressing cells increased (Figure 1B). KLRK1 also reduced the migration distance of cancer cells (Figures 1C, D).

Figure 1. The effect of KLRK1 in lung cancer A549 cell lines. (Zhang Y, et al., 2022)

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