Panoply™ Human KLRK1 Over-expressing Stable Cell Line

Name: Panoply™ Human KLRK1 Over-expressing Stable Cell Line
SKU: CSC-SC008330

Cat.No. : CSC-SC008330 Host Cell: HEK293 (CHO and other cell types are also available)

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-SC008330
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Gene	KLRK1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Size Form	2 × 10^6 cells / vial
Shipping	Dry Ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Case Study

Publications

Q & A

Customer Reviews

Lung cancer is one of the most common malignancies worldwide, resulting in approximately 1.6 million deaths annually. Cancer immune surveillance has been found to play a significant role in lung cancer and may be associated with its prognosis. KLRK1, encoding NKG2D, is a homodimeric lectin-like receptor. However, studies investigating KLRK1 as a lung cancer biomarker are limited. Here, researchers downloaded data from the TCGA database, including patient clinical characteristics and KLRK1 RNAseq information. A total of 1,019 lung cancer patients (407 female and 611 male) were included. The study demonstrated that KLRK1 expression is lower in tumors than in normal tissues. KLRK1 expression is associated with sex, histological grade, stage, T stage, and vital status. Patients with high KLRK1 expression have prolonged overall and recurrence-free survival. Analysis of overall survival in the subgroups of lung adenocarcinoma, stage I/II, elderly patients, and male patients, as well as recurrence-free survival in the subgroups of lung adenocarcinoma, stage I/II, elderly patients, and male patients, revealed significant prognostic value for KLRK1. Lung adenocarcinoma patients with high KLRK1 expression have improved overall and recurrence-free survival. In vitro studies have shown that KLRK1 can inhibit tumor cell proliferation and migration. KLRK1 is an independent prognostic factor, and high KLRK1 expression is associated with improved overall and recurrence-free survival. KLRK1 may serve as a prognostic biomarker for lung adenocarcinoma.

The researchers overexpressed KLRK1 in lung cancer A549 cells to investigate its effects on cells. CCK-8 assays showed that cell proliferation was significantly inhibited in KLRK1-overexpressing cells (Figure 1A). Compared with the control group, the percentage of dead cells (live/dead staining) in KLRK1-overexpressing cells increased (Figure 1B). KLRK1 also reduced the migration distance of cancer cells (Figures 1C, D).

Figure 1. The effect of KLRK1 in lung cancer A549 cell lines. (Zhang Y, et al., 2022)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *