Panoply™ Human KLRB1 Knockdown Stable Cell Line

Name: Panoply™ Human KLRB1 Knockdown Stable Cell Line
SKU: CSC-DC008319
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC008319

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

Inquire for Price

Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC008319
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	KLRB1
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KLRB1 killer cell lectin-like receptor subfamily B, member 1 [ Homo sapiens ]
Gene Symbol	KLRB1
Synonyms	NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
Gene Description	killer cell lectin-like receptor subfamily B, member 1
GeneID	3820
Uni ProtID	Q12918
mRNA Refseq	NM_002258.2
Protein Refseq	NP_002249.1
Chromosome Location	12p13
Function	carbohydrate binding; transmembrane signaling receptor activity;
Pathway	Malaria, organism-specific biosystem; Malaria, conserved biosystem;
MIM	602890

Quick Inquiry

Case Study

Q & A

Customer Reviews

Lung cancer is the most common primary malignant tumor of the lung. As one of the malignant tumors that poses the greatest threat to human health, its incidence has remained high in recent years. Previous studies have shown that KLRB1 is transcriptionally repressed in lung adenocarcinoma and is associated with the prognosis of lung adenocarcinoma. Here, the researchers explored the intrinsic mechanism by which KLRB1 affects the malignant phenotypes of lung adenocarcinoma, such as immune infiltration, proliferation, growth, and metastasis. Studies have shown that KLRB1 expression in lung cancer tissue is lower than that in surrounding healthy tissue. In the KLRB1 low expression group and high expression group, differentially expressed genes were significantly enriched in immune-related pathways. KLRB1 regulates the growth and proliferation of lung adenocarcinoma cells by affecting the MAPK/ERK signaling pathway. KLRB1 expression is associated with the prognosis, immune infiltration, cell migration and proliferation of lung adenocarcinoma (LUAD).

Here, researchers constructed KLRB1 knockdown A549 cells to examine the role of KLRB1 in lung cancer cells. RT-qPCR and Western blot analysis demonstrated that siRNA suppressed KLRB1 expression in A549 cells (Figures 1A and 1B). The researchers assessed the migration and invasion abilities of control (NC) and KLRB1 knockdown A549 cells. Results showed that KLRB1 knockdown cells significantly enhanced their migration and invasion abilities compared with control cells (Figure 1C). These results suggest that KLRB1 depletion increases the metastatic potential of lung cancer cells in vitro. A CF assay was performed to assess the clonogenicity of KLRB1. Results demonstrated that reduced KLRB1 levels significantly enhanced the CF ability of A549 cells (Figure 1D). CCK-8 analysis demonstrated that KLRB1 knockdown significantly increased A549 cell viability (Figure 1E).

Figure 1. Silencing KLRB1 promoted the migration, invasion and proliferation of lung adenocarcinoma cells in vitro. (Xu S, et al., 2024)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *