Panoply™ Human EZH2 Knockdown Stable Cell Line

Name: Panoply™ Human EZH2 Knockdown Stable Cell Line
SKU: CSC-DC005162
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC005162

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC005162
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	EZH2
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	EZH2 enhancer of zeste homolog 2 (Drosophila) [ Homo sapiens ]
Gene Symbol	EZH2
Synonyms	ENX1; EZH1; KMT6; WVS2; ENX-1; KMT6A
Gene Description	enhancer of zeste homolog 2 (Drosophila)
GeneID	2146
Uni ProtID	Q15910
mRNA Refseq	NM_001203249.1
Protein Refseq	NP_001190178.1
Chromosome Location	7q35-q36
Function	DNA binding; chromatin binding; core promoter binding; histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding; sequence-specific DNA binding;
Pathway	Integrated Pancreatic Cancer Pathway, organism-specific biosystem; miRs in Muscle Cell Differentiation, organism-specific biosystem;
MIM	601573

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Enhancer of zeste homolog 2 (EZH2) is a member of the polycomb protein family and an oncogene that is overexpressed in various human cancers. Here, researchers used immunohistochemical staining to find that EZH2 expression levels are associated with poor prognosis in oral squamous cell carcinoma (OSCC) patients. EZH2 overexpression significantly promoted glycolysis, epithelial-mesenchymal transition (EMT), migration, and invasion in OSCC cells. Conversely, EZH2 silencing inhibited glycolysis, EMT, migration, and invasion in OSCC cells. Ectopic overexpression of EZH2 increased STAT3 phosphorylation at pY705 and decreased FoxO1 expression; while inhibiting STAT3 enhanced FoxO1 expression. Furthermore, in a nude mouse xenograft model, EZH2 overexpression led to a significant decrease in FoxO1 mRNA levels. These results suggest that targeting EZH2 may be a potential therapeutic target for OSCC.

To investigate the role of EZH2 in oral squamous cell carcinoma (OSCC) cell migration, invasion, and epithelial-mesenchymal transition (EMT), researchers constructed EZH2 knockdown Cal-27 and Tca8113 cell lines, and confirmed the knockdown effect using Western blot and RT-PCR (Figure 1A). Wound healing assays showed that the cell migration area was reduced by 31.9% and 23% in EZH2 knockdown Cal-27 and Tca8113 cells, respectively (Figure 1B). Compared with the control group, the invasion ability of EZH2 knockdown Cal-27 and Tca8113 cells was reduced by 34.9% and 24%, respectively (Figure 1C). Subsequently, researchers detected EMT-related markers using Western blot and RT-PCR. The results showed that in EZH2 knockdown Cal-27 cells, the protein and mRNA levels of epithelial markers E-cadherin and β-catenin were significantly increased, while the protein and mRNA levels of mesenchymal markers N-cadherin and vimentin were significantly decreased (Figure 1D), which was also confirmed by immunofluorescence staining (Figure 1E). However, no significant difference in apoptosis was detected between the EZH2 knockdown group and the control group (Figure 1F).

Figure 1. EZH2 knockdown inhibits migration, invasion potential and EMT in OSCC cells. (Zheng M, et al., 2019)

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