Cat.No. : CSC-SC003703 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC003703 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | CSF2RB |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CSF2RB colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) [ Homo sapiens ] |
| Gene Symbol | CSF2RB |
| Synonyms | CD131; IL3RB; IL5RB; SMDP5; CDw131 |
| Gene ID | 1439 |
| UniProt ID | P32927 |
| mRNA Refseq | NM_000395.2 |
| Protein Refseq | NP_000386.1 |
| Chromosome Location | 22q13.1 |
| Function | contributes_to interleukin-3 receptor activity; contributes_to interleukin-5 receptor activity; protein binding; receptor activity; |
| Pathway | Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL-3 Signaling Pathway, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem; |
| MIM | 138981 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Invasive intramyocardial injection of mesenchymal stromal cells (MSCs) limits the number of repeat injections and has limited efficacy in treating ischemic heart failure. Here, researchers investigated whether and how overexpression of colony-stimulating factor 2 receptor β subunit (CSF2RB) regulates cardiac homing of MSCs and their cardioprotective effects against ischemic heart failure. Adult mice underwent myocardial ischemia/reperfusion (MI/R) or sham surgery. CSF2 protein expression and secretion were significantly increased in ischemic hearts one day to two weeks after MI/R. Mouse adipose tissue-derived MSCs (ADSCs) were infected with either an adenovirus carrying CSF2RB or a control adenovirus. Intravenously injected ADSCs overexpressing CSF2RB exhibited significantly enhanced cardiac homing compared to ADSCs infected with the control adenovirus. Histological analysis revealed that overexpression of CSF2RB significantly enhanced the pro-angiogenic, anti-apoptotic, and anti-fibrotic effects mediated by ADSCs. More importantly, ADSCs overexpressing CSF2RB significantly improved left ventricular ejection fraction and cardiac systolic/diastolic function in MI/R mice. In vitro experiments showed that CSF2RB overexpression enhanced ADSC migration and reduced hypoxia/reoxygenation-induced apoptosis. STAT5 phosphorylation is a key mechanism by which CSF2RB promotes ADSC migration and inhibits ADSC apoptosis. RNA sequencing and causal analysis showed that CSF2RB overexpression increased the expression of the ubiquitin ligase RNF4. Immunoprecipitation and immunostaining experiments demonstrated that RNF4 binds to phosphorylated STAT5. RNF4 knockdown reduced STAT5 phosphorylation and the anti-apoptotic and pro-migratory effects of ADSCs overexpressing CSF2RB.
Here, researchers investigated the cellular mechanisms by which CSF2RB-overexpressing ADSCs enhance cardiac homing and repair. Because stem cell homing largely depends on their migratory capacity, the researchers first compared the migratory capacity of CSF2RB-overexpressing ADSCs with that of control cells. Wound healing assays and Matrigel-coated Transwell assays demonstrated that CSF2RB-overexpressing ADSCs exhibited significantly improved migratory capacity compared with control cells (Figure 1A-B). Matrix metalloproteinases (MMPs) have previously been reported to play a crucial role in MSC migration. The researchers found that CSF2RB overexpression significantly increased the protein expression of MMP-2, MMP-3, and MMP-9 in ADSCs (Figure 1C). After homing to the infarcted heart, stem cells encounter a harsh ischemic microenvironment, which impairs their retention. Therefore, they evaluated the anti-apoptotic potential of CSF2RB-overexpressing ADSCs. Immunoblotting revealed that after H/R treatment, caspase-3 protein expression was significantly reduced in CSF2RB-overexpressing ADSCs compared with control cells (Figure 1D). After H/R treatment, the number of TUNEL+ nuclei in CSF2RB-overexpressing ADSCs was significantly lower than that in control cells (Figure 1E).
Figure 1. CSF2RB overexpression increased the migration and antiapoptotic effect of ADSCs. (Qi T, et al., 2023)
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