Panoply™ Human CSF2RB Over-expressing Stable Cell Line

Name: Panoply™ Human CSF2RB Over-expressing Stable Cell Line
SKU: CSC-SC003703
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC003703

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC003703
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CSF2RB
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CSF2RB colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) [ Homo sapiens ]
Gene Symbol	CSF2RB
Synonyms	CD131; IL3RB; IL5RB; SMDP5; CDw131
Gene ID	1439
Uni Prot ID	P32927
m RNA Refseq	NM_000395.2
Protein Refseq	NP_000386.1
Chromosome Location	22q13.1
Function	contributes_to interleukin-3 receptor activity; contributes_to interleukin-5 receptor activity; protein binding; receptor activity;
Pathway	Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL-3 Signaling Pathway, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem;
MIM	138981

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Invasive intramyocardial injection of mesenchymal stromal cells (MSCs) limits the number of repeat injections and has limited efficacy in treating ischemic heart failure. Here, researchers investigated whether and how overexpression of colony-stimulating factor 2 receptor β subunit (CSF2RB) regulates cardiac homing of MSCs and their cardioprotective effects against ischemic heart failure. Adult mice underwent myocardial ischemia/reperfusion (MI/R) or sham surgery. CSF2 protein expression and secretion were significantly increased in ischemic hearts one day to two weeks after MI/R. Mouse adipose tissue-derived MSCs (ADSCs) were infected with either an adenovirus carrying CSF2RB or a control adenovirus. Intravenously injected ADSCs overexpressing CSF2RB exhibited significantly enhanced cardiac homing compared to ADSCs infected with the control adenovirus. Histological analysis revealed that overexpression of CSF2RB significantly enhanced the pro-angiogenic, anti-apoptotic, and anti-fibrotic effects mediated by ADSCs. More importantly, ADSCs overexpressing CSF2RB significantly improved left ventricular ejection fraction and cardiac systolic/diastolic function in MI/R mice. In vitro experiments showed that CSF2RB overexpression enhanced ADSC migration and reduced hypoxia/reoxygenation-induced apoptosis. STAT5 phosphorylation is a key mechanism by which CSF2RB promotes ADSC migration and inhibits ADSC apoptosis. RNA sequencing and causal analysis showed that CSF2RB overexpression increased the expression of the ubiquitin ligase RNF4. Immunoprecipitation and immunostaining experiments demonstrated that RNF4 binds to phosphorylated STAT5. RNF4 knockdown reduced STAT5 phosphorylation and the anti-apoptotic and pro-migratory effects of ADSCs overexpressing CSF2RB.

Here, researchers investigated the cellular mechanisms by which CSF2RB-overexpressing ADSCs enhance cardiac homing and repair. Because stem cell homing largely depends on their migratory capacity, the researchers first compared the migratory capacity of CSF2RB-overexpressing ADSCs with that of control cells. Wound healing assays and Matrigel-coated Transwell assays demonstrated that CSF2RB-overexpressing ADSCs exhibited significantly improved migratory capacity compared with control cells (Figure 1A-B). Matrix metalloproteinases (MMPs) have previously been reported to play a crucial role in MSC migration. The researchers found that CSF2RB overexpression significantly increased the protein expression of MMP-2, MMP-3, and MMP-9 in ADSCs (Figure 1C). After homing to the infarcted heart, stem cells encounter a harsh ischemic microenvironment, which impairs their retention. Therefore, they evaluated the anti-apoptotic potential of CSF2RB-overexpressing ADSCs. Immunoblotting revealed that after H/R treatment, caspase-3 protein expression was significantly reduced in CSF2RB-overexpressing ADSCs compared with control cells (Figure 1D). After H/R treatment, the number of TUNEL+ nuclei in CSF2RB-overexpressing ADSCs was significantly lower than that in control cells (Figure 1E).

Figure 1. CSF2RB overexpression increased the migration and antiapoptotic effect of ADSCs. (Qi T, et al., 2023)

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