Panoply™ Human CENPE Over-expressing Stable Cell Line

Centromere-associated protein E (CENPE) has been shown to be overexpressed in various cancers and exerts pro-tumor effects by influencing chromosomal misalignment and mitosis. However, the expression pattern, biological function, and underlying molecular mechanisms of CENPE in clear cell renal cell carcinoma (ccRCC) progression remain largely unknown. Here, researchers measured the expression levels of CENPE in ccRCC and adjacent normal tissue samples using public RNA sequencing data and validated the findings in an independent cohort of ccRCC samples. They found that CENPE was significantly overexpressed in ccRCC tissues and promoted the proliferation and metastatic ability of ccRCC cells and xenograft tumors by regulating the epithelial-mesenchymal transition (EMT) process. Furthermore, bioinformatics analysis and ChIP experiments indicated that the transcription factor CREB1 binds to the promoter region of CENPE and activates its transcription in ccRCC cells. In summary, these findings suggest that the CREB1-CENPE axis is responsible for promoting ccRCC progression in vitro and in vivo, and may serve as a potential therapeutic target for ccRCC.

Due to the high expression level of CENPE, 786O and CAKI-1 cells were used in the following experiments. Researchers constructed CENPE knockdown 786O and CAKI-1 cells (Figure 1a, c) and CENPE overexpression (oe-CENPE) cells (Figure 1b, d). Subsequently, they investigated the role of CENPE in cell proliferation. Knockdown of CENPE significantly reduced the viability of ccRCC cells (Figure 1e, f), decreased the proportion of EdU-positive cells (Figure 1i, j), and reduced the number of colony formations (Figure 1k). Conversely, cell viability (Figure 1g, h) and colony number (Figure 1l) significantly increased in CENPE-overexpressing cells, indicating that CENPE promotes ccRCC cell proliferation.

Figure 1. CENPE promotes the proliferation of ccRCC in vitro. (Jiang H, et al., 2025)