Panoply™ Human AOC3 Over-expressing Stable Cell Line

Amine oxidase copper containing 3 (AOC3) has been reported to play a crucial regulatory role in biological pathways, the immune microenvironment, and cellular function. However, the potential function of AOC3 in cancer remains largely unexplored. Here, researchers comprehensively analyzed the potential function of AOC3 in pan-cancer using multiple online databases and analytical methods, including mutation and differential expression analysis, pathway analysis, and immunoassay. Subsequently, they assessed the function of AOC3 in colorectal cancer (CRC) cells. Pan-cancer analysis revealed that the mutation frequency of AOC3 was highest in patients with embryonal tumors. AOC3 expression was significantly downregulated in most cancers, and differential expression of AOC3 was primarily concentrated in CRC and female tumors. Enrichment analysis indicated that AOC3 is involved in the PPAR signaling pathway. Furthermore, bioinformatics analysis revealed the association between AOC3 and immune cell infiltration in the tumor microenvironment. Cellular experiments confirmed that AOC3 can significantly regulate apoptosis and cell cycle progression in CRC cells. In summary, these studies not only comprehensively analyzed the potential mechanisms of AOC3 in pan-cancer, but also verified the potential regulatory role of AOC3 through CRC cell experiments.

Here, the HTC116 cell apoptosis experiment showed that, compared with control cells, the apoptosis rate of AOC3-overexpressing HTC116 cells was significantly increased (Figure 1A-B).

Figure 1. Comparison of apoptosis rate between two groups (HCT116 + NC group and HTC116 + AOC3-OE group). (Wang G, et al., 2025)