Panoply™ Human ACVR2B Knockdown Stable Cell Line

Name: Panoply™ Human ACVR2B Knockdown Stable Cell Line
SKU: CSC-DC000218

Cat.No. : CSC-DC000218

Host Cell: HEK293 (Hela and other cell types are also available) Validation: Real-Time RCR

Inquire for Price

Cell Line Information

Safety and Packaging

Cat. No.	CSC-DC000218
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Gene	ACVR2B
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Size Form	>1 × 10^6 cells / vial
Shipping	Dry Ice
Storage	Liquid Nitrogen

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Case Study

Publications

Q & A

Customer Reviews

Circular RNAs (circRNAs) play regulatory roles in cerebrovascular disease. Human brain microvascular endothelial cells (HBMECs) are involved in cerebrovascular dysfunction in ischemic stroke. Here, researchers investigated the role of circ_0000566 in oxygen-glucose deprivation/reoxygenation (OGD/R)-induced HBMECs. Results showed that circ_0000566 and ACVR2B were highly expressed, whereas miR-18a-5p was downregulated in OGD/R-treated HBMECs. OGD/R treatment promoted apoptosis and inflammation in HBMECs and inhibited cell viability, whereas silencing circ_0000566 mitigated these effects. Circ_0000566, acting as a sponge for miR-18a-5p, was involved in OGD/R-induced HBMEC damage. ACVR2B is a direct target of miR-18a-5p. ACVR2B overexpression may abrogate the inhibitory effect of miR-18a-5p on OGD/R-treated HBMECs injury. Circ_0000566 sponged miR-18a-5p to regulate OGD/R-induced HBMECs injury via regulating ACVR2B expression.

Here, researchers investigated the role of ACVR2B in OGD/R-induced HBMEC injury. Transfection of HBMECs with si-ACVR2B significantly reduced ACVR2B protein expression (Figure 1A). Furthermore, OGD/R-induced ACVR2B expression was inhibited by si-ACVR2B transfection (Figure 1B). ACVR2B knockdown in OGD/R-induced HBMECs increased cell viability and Bcl-2 protein levels, while inhibiting LDH release, apoptosis rate, and protein expression of Bax, c-caspase 3, and c-caspase 9 (Figures 1C-J). Furthermore, ACVR2B knockdown also reduced IL-1β, IL-6, and TNF-α levels in OGD/R-induced HBMECs (Figures 1K-M). These data confirm that ACVR2B knockdown alleviates OGD/R-induced HBMEC injury.

Figure 1. ACVR2B regulated OGD/R-stimulated HBMEC damage. (Liu D, et al., 2023)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *