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MIR466a-pCMV-MIR

For research use only. Not intended for any clinical use.
Cat.No.
MIPM2228
Description
Expression plasmid for mouse microRNA Mir466a. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.
Reporter
GFP
Size/Form
The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.
Species
Mouse
Vector Type
plasmid
miRNA Information
Precursor Accession No.
MI0002401
Precursor Name
mmu-mir-466a
Precursors sequence
UAUAUGUGUUUAUGUGUGUGUACAUGUACAUAUGU GAAUAUGAUAUCCAUAUACAUACACGCACACAUAA GAC
Precursors Length
73 bp
Gene Description
Mus musculus miR-466a stem-loop
Gene Species
Mouse
Mature miRNA-1 Accession No.
MIMAT0004759
Mature miRNA-1 Name
mmu-miR-466a-5p
Mature miRNA-1 sequence
UAUGUGUGUGUACAUGUACAUA
Mature miRNA-2 Accession No.
MIMAT0002107
Mature miRNA-2 Name
mmu-miR-466a-3p
Mature miRNA-2 sequence
UAUACAUACACGCACACAUAAGA

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