MIR125a-pCMV-MIR

Name: MIR125a-pCMV-MIR
SKU: MIPM2011

For research use only. Not intended for any clinical use.

Cat.No.

MIPM2011

Description

Expression plasmid for mouse microRNA Mir125a. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.

Reporter

GFP

Size/Form

The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.

Species

Mouse

Vector Type

plasmid

miRNA Information

Precursor Accession No.

MI0000151

Precursor Name

mmu-mir-125a

Precursors sequence

CUGGGUCCCUGAGACCCUUUAACCUGUGAGGACGU CCAGGGUCACAGGUGAGGUUCUUGGGAGCCUGG

Precursors Length

68 bp

Gene Description

Mus musculus miR-125a stem-loop

Gene Species

Mouse

Mature miRNA-1 Accession No.

MIMAT0000135

Mature miRNA-1 Name

mmu-miR-125a-5p

Mature miRNA-1 sequence

UCCCUGAGACCCUUUAACCUGUGA

Mature miRNA-2 Accession No.

MIMAT0004528

Mature miRNA-2 Name

mmu-miR-125a-3p

Mature miRNA-2 sequence

ACAGGUGAGGUUCUUGGGAGCC

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