Cat.No. : AD00276Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00276Z |
| Target Gene | WNT5A |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | WNT5A |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
microRNA (miR)-374a plays a crucial role in cancer progression by promoting metastasis and proliferation of multiple malignancies. Here, researchers investigated whether miR-374a affects the progression of bladder cancer and investigated the underlying mechanisms. Low levels of miR-374a were associated with poor prognosis in bladder cancer patients with distant metastasis by in silico analysis. WNT5A was a direct target of miR-374a in two bladder cancer cell lines. In T24 and TCCSUP human bladder cancer cells, miR-374a mimics abolished the metastatic potential and invasiveness of bladder cancer cells by downregulating WNT5A; whereas the opposite was observed with miR-374a inhibitors. In addition, miR-374a treatment reduced the phosphorylation and nuclear translocation of β-catenin. Cisplatin treatment significantly increased the rate of apoptosis. The expression levels of cancer stem cell-related proteins were reduced in cells pretreated with miR-374a mimics. Therefore, these studies indicate that miR-374a can improve the tumor biological behavior of bladder cancer cells, suggesting that miR-374a may be a new small molecule therapeutic target.
Here, after the researchers introduced an adenoviral vector overexpressing WNT5A (Ad-WNT5A) into T24 cells, WNT5A transcripts were significantly upregulated (Figure 1A). Functionally, overexpression of WNT5A exacerbated the metastasis and invasiveness of T24 cells in wound healing and Transwell assays (Figure 1B-D). In addition, treatment with miR-374a mimics significantly abolished the metastatic capacity and invasiveness of T24 cells (Figure 1B-D). Therefore, both miR-374 and WNT5A can alter bladder cell biology. To determine whether the effects of miR-374a on cancer cell biology are achieved through WNT5A downregulation, T24 cells overexpressing WNT5A were treated with miR-374a mimics. First, miR-374a mimics significantly abolished the increase in WNT5A (Figure 1B-D). Second, the increased metastasis and invasiveness of T24 cells induced by WNT5A overexpression were also inhibited (Figure 1B-D). Similar results were observed in TCCSUP cells.
Figure 1. miR-374a mimic decreases metastatic and invasive abilities in bladder cancer cells via degeneration of WNT5A. (Chen X, et al., 2018)
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The purity of these Human WNT5A adenoviral particles was exceptional, resulting in minimal cellular toxicity in my HEK293 cells. This allowed for cleaner experimental outcomes and reliable data interpretation.
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