Cat.No. : CSC-RG0055 Host Cell: CHO
| Cat. No. | CSC-RG0055 |
| Background | This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. |
| Gene | VIPR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | VIPR1, HVR1, RDC1, VPAC1, VPAC1R, V1RG, VIPR, PACAP-R2, PACAP-R-2, FLJ41949 |
| Host Cell | CHO |
| Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human VIPR1-SNAP Stable Cell Line-CHO serves as a controlled environment for researchers to investigate the activity of the VIPR1 receptor and its role in signal transduction. By expressing VIPR1 with a SNAP tag in CHO cells, scientists can utilize the specificity of the SNAP tag for protein labeling and detection through SNAP-tag technology, enabling the study of VIPR1's interaction partners, localization, and signaling pathways.
A: The SNAP tag is typically designed to be small enough to minimize its impact on the function of the target protein. In drug screening, the introduction of the SNAP tag is unlikely to significantly alter the binding pocket or drug binding characteristics of VIPR1. However, to ensure that the SNAP tag does not affect the results of drug screening, researchers usually perform control experiments comparing the drug binding and function of VIPR1 with and without the SNAP tag.
A: In HTS, the Human VIPR1-SNAP Stable Cell Line-CHO can screen for potential agonists and antagonists of VIPR1 through its stable VIPR1 expression. By monitoring changes in signal transduction following VIPR1 activation, compounds that enhance or inhibit VIPR1 activity can be identified. This approach allows for the rapid identification of candidate drug molecules, providing a foundation for subsequent pharmacological and clinical research.
A: To ensure that the SNAP tag does not interfere with the natural physiological processes of cells, researchers need to perform a series of validation experiments. This includes comparing the physiological responses and signal transduction of VIPR1 with and without the SNAP tag. Additionally, the impact of the SNAP tag on basic cellular functions such as cell growth, differentiation, and apoptosis should be assessed. Through these validation steps, the introduction of the SNAP tag can be minimized to have the least impact on cell behavior.
A: The introduction of the SNAP tag may affect the phosphorylation status of VIPR1, especially if the tag is near the phosphorylation sites. However, by choosing the appropriate tag location and performing validation experiments, this impact can be minimized. For example, by comparing the phosphorylation levels of VIPR1 with and without the SNAP tag, the effect of the SNAP tag on phosphorylation events can be assessed.
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