Cat.No. : AD00271Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00271Z |
| Target Gene | TSHR |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | TSHR |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Graves' orbitopathy (GO) is an autoimmune orbital disease. Dysfunction of the gut microbiota plays a crucial role in autoimmune diseases such as Graves' disease (GD) and GO. Here, researchers aimed to study the changes in the gut microbiota in GD/GO using a mouse model. The murine model of GD/GO was established by the challenge of adenovirus expressing thyroid-stimulating hormone (TSH) receptor (TSHR) (Ad-TSHR). The GD/GO model was successfully established, showing widened eyelids, proptosis and conjunctival redness, severe inflammatory infiltration between the thyroid gland and the extraocular muscle space, hypertrophy of the extraocular muscles, elevated thyroxine (T4) and decreased TSH, and positive staining for CD34, CD40, collagen I, and α-SMA. A total of 222 operational taxonomic units (OUTs) overlapped between the Ad-NC and Ad-TSHR group mice. The microbial composition of the samples in both groups was dominated by Bacteroidia and Clostridia, and the Ad-NC group had a significantly lower content of Bacteroidia and higher content of Clostridia. The results of KEGG orthologous homology analysis showed that dehydrogenase, aspartate, bile acid, chalcone synthase, acetyltransferase, glutamyl cyclotransferase, glycogenin, and 1-phosphatidylinositol-4-phosphate 5-kinase were different between the two groups. The results of enzyme commission (EC) analysis showed that several dehydrogenases, oxidases, and sulfur oxygen/reductases were different between the two groups. These research results can lay a solid experimental foundation for developing personalized treatment regimens for GD patients according to the individual gut microbiota.
First, the mouse GD/GO model was established by Ad-TSHR challenge. The mice were randomly divided into groups (Ad-TSHR, Ad-NC, and blank groups). The mice in the Ad-TSHR group were inoculated with TSHR adenovirus (Figure 1A). The morphology of mice in each group was observed at week 15, and representative images (lateral and front appearances) were taken. It was observed that mice in the Ad-TSHR group exhibited broadened eyelid, exophthalmos, and conjunctive redness (Figure 1B). Orbital and thyroid histopathological examination showed that the inflammatory infiltration between the thyroid gland and the extraocular muscle space of the Ad-TSHR group mice was severe, the extraocular muscle space was widened, and the vascular hyperplasia was more obvious than that of the blank group and the Ad-NC group. In addition, the degree of extraocular muscle hypertrophy in the Ad-TSHR group was better than that in the blank group (Figure 1C).
Figure 1. The murine model of GD/GO was established by immunization with thyroid-stimulating hormone receptor expressing adenovirus (Ad-TSHR). (Li Y, et al., 2023)
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Creative Biogene consistently delivers high-quality viral vectors. Their Human TSHR adenoviral particles met all expectations, and the provided documentation was comprehensive.
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