Human SSTR1 Stable Cell Line-CHO-K1

Name: Human SSTR1 Stable Cell Line-CHO-K1
SKU: CSC-RG0068
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0068

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0068
Target Gene	SSTR1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuSSTR1
Alias	SSTR1, SS1R, SS1-R, SRIF-2, SS-1-R
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SSTR1 somatostatin receptor 1 [ Homo sapiens ]
Gene Symbol	SSTR1
Synonyms	SS1R; SS1-R; SRIF-2; SS-1-R
Gene Description	somatostatin receptor 1
Gene ID	6751
Uni Prot ID	P30872
m RNA Refseq	NM_001049.2
Protein Refseq	NP_001040.1
Chromosome Location	14q13
Function	somatostatin receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	182451

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Background

Q & A

Customer Reviews

Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. The protein encoded by this gene is a member of the superfamily of somatostatin receptors having seven transmembrane segments. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This somatostatin receptor has greater affinity for somatostatin-14 than -28.

Customer Q&As

What genetic engineering techniques are typically used to construct the Human SSTR1 Stable Cell Line-CHO?

A: The construction of the Human SSTR1 Stable Cell Line-CHO typically involves steps such as gene cloning, vector construction, transfection, selection, and identification. Initially, the SSTR1 gene is cloned into an appropriate expression vector, which is then introduced into CHO cells via transfection. Subsequently, cells that have successfully integrated and express SSTR1 are selected using antibiotic selection or other selection markers. Finally, the stability and functionality of the cell line are confirmed through functional and expression level assays.

What is the role of the Human SSTR1 Stable Cell Line-CHO in the study of neurodegenerative diseases?

A: The role of the Human SSTR1 Stable Cell Line-CHO in the study of neurodegenerative diseases is primarily to simulate the neuronal environment under disease conditions and investigate the mechanisms of action of SSTR1 in these diseases. For instance, in the study of Parkinson's disease and Alzheimer's disease, this cell line can be used to test the effects of potential drugs on the modulation of SSTR1 and how these modulations affect neuronal survival and function.

How can the culture conditions of the Human SSTR1 Stable Cell Line-CHO be optimized to enhance SSTR1 expression and activity?

A: To optimize the culture conditions of the Human SSTR1 Stable Cell Line-CHO, researchers need to consider various factors, including the composition of the culture medium, temperature, pH, CO2 concentration, and the addition of nutrients. Additionally, specific growth factors, hormones, or small molecule compounds can be used to enhance SSTR1 expression and activity. Regular monitoring of cell growth status and SSTR1 expression levels, as well as stability testing of the cell line, are also key to ensuring optimized culture conditions.

What are the potential applications of the Human SSTR1 Stable Cell Line-CHO in drug development?

A: The potential applications of the Human SSTR1 Stable Cell Line-CHO in drug development include drug screening, pharmacological studies, pharmacokinetic studies, and drug safety assessment. This cell line can be used to screen potential drug candidates targeting SSTR1, evaluate the affinity and selectivity of these drugs for SSTR1, and study the metabolic processes and potential toxic effects of drugs within the cells.

What is the role of the Human SSTR1 Stable Cell Line-CHO in studying the endocrine system?

A: The role of the Human SSTR1 Stable Cell Line-CHO in studying the endocrine system is primarily to investigate the mechanisms by which somatostatin and its receptor SSTR1 regulate hormone secretion and metabolism. This cell line can help scientists understand the role of SSTR1 in maintaining endocrine balance and its potential therapeutic potential in endocrine-related diseases such as diabetes and thyroid disorders.

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