Human SRF Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLa

Name: Human SRF Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLa
SKU: CSC-RR0035
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0035

Host Cell : HeLa Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-RR0035
Target Gene	SRF
Gene Species	Homo sapiens (Human)
Abbr	HeLa-HuSRF Prom/Luciferase/Hyg
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SRF serum response factor (c-fos serum response element-binding transcription factor) [ Homo sapiens ]
Gene Symbol	SRF
Synonyms	MCM1
Gene Description	serum response factor (c-fos serum response element-binding transcription factor)
Gene ID	6722
Uni Prot ID	P11831
m RNA Refseq	NM_003131.2
Protein Refseq	NP_003122.1
Chromosome Location	6p21.1
Function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; contributes_to RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; RNA polymerase II transcription factor binding transcription factor activity; chromatin DNA binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; serum response element binding; transcription factor binding;
Pathway	Coregulation of Androgen receptor activity, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Heart Development, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem;
MIM	600589

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Serum response factor (SRF) is a widely expressed transcription factor involved in the expression of genes linked to cellular growth and muscle differentiation. Its target genes include cellular immediate early genes such as c-fos and structural cytoskeletal proteins, such as actins, myosins, tropomyosin, and vinculin. Serum-induced transcriptional activation of immediate early genes involves the formation of a ternary complex of SRF with a 62 kDa ternary complex factor (TCF) which binds to the serum response element (SRE). SRF is also involved in vascular smooth muscle differentiation by regulating the expression of several contractile and cytoskeletal genes. It is widely accepted that normal smooth muscle cells are subverted to one of growth and excess matrix production in various disease.

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