Human SRF Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLa

Name: Human SRF Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLa
SKU: CSC-RR0035

Cat.No. : CSC-RR0035 Host Cell: HeLa

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0035
Gene	SRF
Gene Species	Homo sapiens (Human)
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Serum response factor (SRF) is a widely expressed transcription factor involved in the expression of genes linked to cellular growth and muscle differentiation. Its target genes include cellular immediate early genes such as c-fos and structural cytoskeletal proteins, such as actins, myosins, tropomyosin, and vinculin. Serum-induced transcriptional activation of immediate early genes involves the formation of a ternary complex of SRF with a 62 kDa ternary complex factor (TCF) which binds to the serum response element (SRE). SRF is also involved in vascular smooth muscle differentiation by regulating the expression of several contractile and cytoskeletal genes. It is widely accepted that normal smooth muscle cells are subverted to one of growth and excess matrix production in various disease.

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