Cat.No. : AD00245Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00245Z |
| Target Gene | RGS2 |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | RGS2 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | RGS2 regulator of G-protein signaling 2, 24kDa [ Homo sapiens ] |
| Gene Symbol | RGS2 |
| Synonyms | RGS2; regulator of G-protein signaling 2, 24kDa; G0S8, regulator of G protein signalling 2, 24kD , regulator of G protein signalling 2, 24kDa; regulator of G-protein signaling 2; G0/G1 switch regulatory protein 8; cell growth-inhibiting protein 31; G0 to G1 switch regulatory 8, 24kD; cell growth-inhibiting gene 31 protein; G0S8; |
| Gene ID | 5997 |
| UniProt ID | P41220 |
| mRNA Refseq | BC007049 |
| Chromosome Location | 1q31 |
| Function | GTPase activator activity; calmodulin binding; protein binding; |
| Pathway | Calcium Regulation in the Cardiac Cell, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem; |
| MIM | 600861 |
Pirfenidone has recently been approved for the treatment of idiopathic pulmonary fibrosis. However, the therapeutic dose of pirfenidone is very high, resulting in side effects that limit its dose and therapeutic efficacy. Understanding the molecular mechanism of action of pirfenidone may improve its safety and efficacy. Here, researchers identified Regulator of G-protein Signaling 2 (RGS2) as an early gene induced by pirfenidone. Treatment with pirfenidone significantly increased RGS2 mRNA and protein expression in a human fetal lung fibroblast cell line and in primary lung fibroblasts isolated from patients with or without idiopathic pulmonary fibrosis. Pirfenidone treatment or direct overexpression of recombinant RGS2 in human lung fibroblasts inhibited the profibrotic effects of thrombin, whereas the absence of RGS2 exacerbated bleomycin-induced lung fibrosis and mortality in mice. Pirfenidone treatment attenuated bleomycin-induced lung fibrosis in wild-type mice but not in RGS2 knockout mice. Thus, endogenous RGS2 has an anti-fibrotic function. Upregulated RGS2 contributes significantly to the antifibrotic effect of pirfenidone.
The serine protease thrombin activates the Gq-coupled protease-activated receptor 1 (PAR1), promoting fibroblast proliferation and differentiation to a myofibroblast phenotype, leading to the development of pulmonary fibrosis. Previous studies have shown that thrombin-stimulated proliferation is dependent on PAR1-mediated increases in [Ca2+]i in multiple cell types. Because RGS2 can act as a selective modulator of Gq-mediated signaling, the researchers investigated the effect of RGS2 expression on thrombin-induced increases in [Ca2+]i in HFL1 cells. RGS2 protein was increased by about 6-fold in HFL1 cells with adenovirus-expressing RGS2 and mCherry reporter in comparison to control cells with the mCherry-expressing adenovirus alone (Figure 1a). Thrombin induced a dose-dependent increase in [Ca2+]i in control HFL1 cells expressing mCherry alone (Figure 1b). Compared with these control cells, overexpression of RGS2 significantly attenuated the thrombin (1 U/ml)-induced increase in [Ca2+]i in HFL1 cells from 3.75 ± 0.07 to 2.31 ± 0.05 (Figure 1c).
Figure 1. Increase of RGS2 attenuates thrombin-induced increase of [Ca2+]i in HFL1 cells. (Xie Y, et al., 2016)
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