Cat.No. : AD00235Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00235Z |
| Target Gene | PRDM16 |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | PRDM16 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | PRDM16 PR domain containing 16 [ Homo sapiens ] |
| Gene Symbol | PRDM16 |
| Synonyms | MEL1; PFM13 |
| Gene ID | 63976 |
| UniProt ID | Q9HAZ2 |
| mRNA Refseq | NM_022114.3 |
| Protein Refseq | NP_071397.3 |
| Chromosome Location | 1p36.23-p33 |
| Function | SMAD binding; protein binding; sequence-specific DNA binding; transcription coactivator activity; zinc ion binding; |
| MIM | 605557 |
Acute kidney injury (AKI) is a major public health problem. Sepsis accounts for more than 50% of AKI cases in ICUs. PRD1-BF1-RIZ1 homeodomain protein 16 (PRDM16) can inhibit the progression of diabetic nephropathy (DKD). However, its exact role and regulatory mechanisms in sepsis-induced AKI remain unclear. Here, researcher show that lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) trigger PRDM16 expression in Boston University mouse proximal tubule (BUMPT) cells and mouse kidney, respectively. Mechanistically, PRDM16 associates with the promoter region of nuclear factor-erythroid 2-related factor-2 (NRF2) and enhances its expression, which subsequently enhances the expression of glutathione peroxidase 4 (GPX4). Notably, these observations were confirmed in human renal tubular epithelial (HK-2) cells. Furthermore, ablation of PRDM16 in the proximal tubules of mouse kidneys suppressed the expression of NRF2 and GPX4, leading to a decreased glutathione (GSH)/oxidized glutathione (GSSG) ratio, increased Fe2+ and reactive oxygen species (ROS) generation, exacerbated ferroptosis, and progression of AKI. In contrast, PRDM16 knock-in exhibited the opposite effect. Finally, adenovirus (ADV)-PRDM16 plasmid or poly(lactide-glycolic acid) (PLGA)-encapsulated formononetin not only attenuated sepsis-induced AKI, but also liver, heart, and lung damage. In conclusion, PRDM16 prevents sepsis-induced multi-organ damage, including AKI, by inhibiting ferroptosis through the NRF2/GPX4 axis or GPX4.
To investigate the effects of PRDM16 on various organs including the kidney, adenovirus (ADV)-PRDM16 plasmid was injected via tail vein three days before the CLP model, and control mice received saline followed by CLP 18 h later. H&E staining of tissue sections showed that PRDM16 overexpression significantly ameliorated CLP-induced renal tubules (Figure 1A), alveoli (Figure 1B), myocardium (Figure 1C), and liver (Figure 1D) damage. Immunoblot analysis showed that PRDM16 overexpression significantly increased the protein levels of PRDM16, NRF2, and GPX4 in kidney, lung, heart, and liver tissues after sham surgery and CLP, but significantly decreased the protein levels of COX2 and NOX1. In conclusion, PRDM16 overexpression alleviates sepsis-induced multi-organ damage by upregulating the NRF2/GPX4 axis.
Figure 1. ADV-PRDM16 plasmid alleviates sepsis-induced multi-organ damage in mice through the NRF2/GPX4 axis. (Zheng Q, et al., 2024)
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Creative Biogene has become our go-to for adenoviral constructs. Their PRDM16 particles are well-packaged, high-titer, and deliver every time.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
