Cat.No. : AD00227Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00227Z |
| Target Gene | PGF |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | PGF |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Here, the researchers investigated the effects of placental growth factor (PGF) overexpression and hyperoxia on lung development in neonatal rats and determined whether anti-PGF antibodies could improve hyperoxia-mediated lung development disorders in neonatal rats. After 7 days of normoxic culture, Normoxia neonatal rats were intraperitoneally or intratracheally injected with saline, adenovirus negative control (Ad-NC), or adenovirus-PGF (Ad-PGF) to form Normoxia groups, Normoxia + Ad-NC groups, and Normoxia + Ad-PGF groups. Hyperoxic neonatal rats were intraperitoneally injected with saline or anti-PGF antibodies to form Hyperoxia groups and Hyperoxia + anti-PGF groups. The results showed that the levels of PGF and its receptor Flt-1 in the lung tissues of neonatal rats in the Normoxia + Ad-PGF group and the Hyperoxia group were significantly increased. PGF overexpression in these groups led to lung injury in neonatal rats, and anti-PGF antibody treatment could significantly cure the lung injury caused by hyperoxia. In addition, PGF overexpression significantly increased TNF-α and Il-6 levels in bronchoalveolar lavage fluid (BAL) in both Normoxia + Ad-PGF and Hyperoxia groups. Immunohistochemical analysis showed that PGF overexpression and hyperoxia treatment significantly increased the expression of angiogenic marker CD34. However, its expression was significantly reduced after administration of anti-PGF antibody (compared with the hyperoxia control group). In conclusion, PGF overexpression impairs lung development in neonatal rats, while inhibition of its expression using anti-PGF antibody improves lung development.
Here, the researchers took lung tissues of newborn rats for IHC detection of CD34 expression, and the results showed that the CD34 expression level in lung tissues of rats in the Normoxia + Ad-PGF group was significantly higher than that in the Normoxia + Ad-NC group (Figure 1). In addition, the CD34 expression level in lung tissues of rats in the hyperoxia group was higher than that in the Normoxia group, while the CD34 expression level in lung tissues of rats in the Hyperoxia + anti-PGF group was significantly lower than that in the Hyperoxia group.
Figure 1. CD34 expression was detected by immunohistochemistry in lung tissues from newborn rats of each indicated group (n=10). (Zhang Z, et al., 2020)
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Successfully used these adenoviral particles in mouse models. The in vivo expression was strong and stable, making them ideal for translational studies.
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