Human OPRM1-SNAP Stable Cell Line-CHO

Name: Human OPRM1-SNAP Stable Cell Line-CHO
SKU: CSC-RG0052
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0052

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0052
Background	This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents such a s beta-endorphn and enkephalins. The NM_001008503.1:c.118A>G allele had been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene.
Target Gene	OPRM1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuOPRM1-SNAP
Alias	OPRM1, MOR1, MOP, hMOP, M-OR-1, MOR, LMOR, OPRM, KIAA0403
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	OPRM1 opioid receptor, mu 1 [ Homo sapiens ]
Gene Symbol	OPRM1
Synonyms	MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
Gene Description	opioid receptor, mu 1
Gene ID	4988
Uni Prot ID	P35372
m RNA Refseq	NM_001008503.1
Protein Refseq	NP_001008503.2
Chromosome Location	6q24-q25
Function	G-protein alpha-subunit binding; G-protein coupled receptor activity; beta-endorphin receptor activity; morphine receptor activity; protein binding; voltage-gated calcium channel activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G-protein activation, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; IL4-mediated signaling events, organism-specific biosystem;
MIM	600018

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Customer Q&As

What is the significance of the Human OPRM1-SNAP Stable Cell Line-CHO in studying opioid receptor function?

A: The Human OPRM1-SNAP Stable Cell Line-CHO (Chinese Hamster Ovary) is crucial for studying the function of the human opioid receptor mu-1 (OPRM1). This cell line allows researchers to express and study the receptor in a controlled environment, which is essential for understanding its role in pain perception, addiction, and the development of analgesic drugs. The SNAP tag enables the visualization and tracking of the receptor's movement within the cell, providing insights into its trafficking and signaling mechanisms.

How does the Human OPRM1-SNAP Stable Cell Line-CHO contribute to the development of new analgesic drugs?

A: The Human OPRM1-SNAP Stable Cell Line-CHO serves as a model system for drug screening and development. By expressing the human OPRM1 receptor, researchers can test the efficacy and safety of potential analgesic compounds. The SNAP tag allows for real-time monitoring of drug-receptor interactions, which can inform the design of more effective and safer pain-relief medications.

How does the Human OPRM1-SNAP Stable Cell Line-CHO differ from other opioid receptor cell lines?

A: The Human OPRM1-SNAP Stable Cell Line-CHO is unique in that it expresses the human OPRM1 receptor with a SNAP tag, which allows for the visualization of the receptor's subcellular localization and trafficking. This feature is not common in other opioid receptor cell lines, which may not have the same level of detail in studying receptor dynamics.

How does the Human OPRM1-SNAP Stable Cell Line-CHO facilitate the study of opioid receptor signaling pathways?

A: The Human OPRM1-SNAP Stable Cell Line-CHO enables researchers to study the signaling pathways activated by the OPRM1 receptor. By monitoring the SNAP-tagged receptor's interactions with downstream signaling molecules, researchers can elucidate the complex network of intracellular signaling events that occur upon opioid binding.

How does the Human OPRM1-SNAP Stable Cell Line-CHO aid in the study of opioid tolerance and dependence?

A: The Human OPRM1-SNAP Stable Cell Line-CHO can be utilized to investigate the molecular mechanisms underlying opioid tolerance and dependence. By chronically exposing the cells to opioids and monitoring changes in receptor expression and function, researchers can gain insights into how the receptor adapts to prolonged drug exposure, which is a key aspect of addiction.

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