Cat.No. : CSC-RG0052 Host Cell: CHO
| Cat. No. | CSC-RG0052 |
| Background | This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents such a s beta-endorphn and enkephalins. The NM_001008503.1:c.118A>G allele had been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene | OPRM1 |
| Gene Species | Homo sapiens (Human) |
| Alias | OPRM1, MOR1, MOP, hMOP, M-OR-1, MOR, LMOR, OPRM, KIAA0403 |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human OPRM1-SNAP Stable Cell Line-CHO (Chinese Hamster Ovary) is crucial for studying the function of the human opioid receptor mu-1 (OPRM1). This cell line allows researchers to express and study the receptor in a controlled environment, which is essential for understanding its role in pain perception, addiction, and the development of analgesic drugs. The SNAP tag enables the visualization and tracking of the receptor's movement within the cell, providing insights into its trafficking and signaling mechanisms.
A: The Human OPRM1-SNAP Stable Cell Line-CHO serves as a model system for drug screening and development. By expressing the human OPRM1 receptor, researchers can test the efficacy and safety of potential analgesic compounds. The SNAP tag allows for real-time monitoring of drug-receptor interactions, which can inform the design of more effective and safer pain-relief medications.
A: The Human OPRM1-SNAP Stable Cell Line-CHO is unique in that it expresses the human OPRM1 receptor with a SNAP tag, which allows for the visualization of the receptor's subcellular localization and trafficking. This feature is not common in other opioid receptor cell lines, which may not have the same level of detail in studying receptor dynamics.
A: The Human OPRM1-SNAP Stable Cell Line-CHO enables researchers to study the signaling pathways activated by the OPRM1 receptor. By monitoring the SNAP-tagged receptor's interactions with downstream signaling molecules, researchers can elucidate the complex network of intracellular signaling events that occur upon opioid binding.
A: The Human OPRM1-SNAP Stable Cell Line-CHO can be utilized to investigate the molecular mechanisms underlying opioid tolerance and dependence. By chronically exposing the cells to opioids and monitoring changes in receptor expression and function, researchers can gain insights into how the receptor adapts to prolonged drug exposure, which is a key aspect of addiction.
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