Cat.No. : CSC-RG0063 Host Cell: CHO-K1
| Cat. No. | CSC-RG0063 |
| Background | The protein encoded by this gene is a G protein-coupled receptor whose expression can be induced by phytohemagglutinin. The encoded integral membrane protein is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ. This gene may be involved in the regulation of numerous brain activities, particularly instinctive and emotional behaviors. A promoter for this gene also functions as a promoter for another gene, regulator of G-protein signalling 19 (RGS19), located on the opposite strand. Three transcript variants encoding the same protein have been found for this gene. |
| Gene | OPRL1 |
| Gene Species | Homo sapiens (Human) |
| Alias | OPRL1, KOR 3, NOCIR, OOR, ORL1, KOR-3, MGC34578 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Common genetic engineering techniques used in constructing the Human OPRL1 Stable Cell Line-CHO include gene cloning, vector construction, transfection, and selection. Initially, the OPRL1 gene is cloned from the human genome, then inserted into a vector suitable for CHO cell expression. Subsequently, the vector is introduced into CHO cells via transfection, and finally, stable OPRL1-expressing cell clones are selected through antibiotic selection or other selection strategies.
A: The role of the Human OPRL1 Stable Cell Line-CHO in studying the addictive properties of opioid drugs is primarily to provide a platform that simulates human receptor activity for assessing drug affinity, agonist or antagonist activity, and drug-induced signaling pathways. This information is crucial for understanding how drugs affect opioid receptors in the brain and for designing drugs with lower potential for addiction.
A: To optimize the Human OPRL1 Stable Cell Line-CHO for increased application efficiency in drug development, various strategies can be employed, such as improving cell culture conditions, optimizing gene expression levels, developing high-throughput screening methods, and implementing strict quality control processes. These measures help enhance the stability of the cell line, increase the accuracy and efficiency of drug screening, and thus accelerate the discovery and development of new drugs.
A: The Human OPRL1 Stable Cell Line-CHO primarily serves as a model system in drug metabolism research to study the metabolic processes of drugs within the body. Using this cell line, scientists can simulate drug metabolism in the liver, assess the metabolic stability of drugs, and investigate the mechanisms of action of drug-metabolizing enzymes. This is crucial for predicting the pharmacokinetic properties of drugs and potential toxic reactions.
A: The potential applications of the Human OPRL1 Stable Cell Line-CHO in drug safety assessment include evaluating the potential toxicity of drugs to opioid receptors, studying drug-induced cellular stress responses, and predicting potential side effects of drugs in the body. This cell line can help scientists identify potential safety risks at an early stage, allowing for the implementation of preventive measures during the drug development process.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
