Human OPRL1 Stable Cell Line-CHO-K1

Name: Human OPRL1 Stable Cell Line-CHO-K1
SKU: CSC-RG0063
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0063

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0063
Background	The protein encoded by this gene is a G protein-coupled receptor whose expression can be induced by phytohemagglutinin. The encoded integral membrane protein is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ. This gene may be involved in the regulation of numerous brain activities, particularly instinctive and emotional behaviors. A promoter for this gene also functions as a promoter for another gene, regulator of G-protein signalling 19 (RGS19), located on the opposite strand. Three transcript variants encoding the same protein have been found for this gene.
Target Gene	OPRL1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuOPRL1
Alias	OPRL1, KOR 3, NOCIR, OOR, ORL1, KOR-3, MGC34578
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens opiate receptor-like 1 DNA.
Gene Symbol	OPRL1
Synonyms	OOR, ORL1, KOR-3, NOCIR
m RNA Refseq	BC038433

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What genetic engineering techniques are typically used to construct the Human OPRL1 Stable Cell Line-CHO?

A: Common genetic engineering techniques used in constructing the Human OPRL1 Stable Cell Line-CHO include gene cloning, vector construction, transfection, and selection. Initially, the OPRL1 gene is cloned from the human genome, then inserted into a vector suitable for CHO cell expression. Subsequently, the vector is introduced into CHO cells via transfection, and finally, stable OPRL1-expressing cell clones are selected through antibiotic selection or other selection strategies.

What is the role of the Human OPRL1 Stable Cell Line-CHO in studying the addictive properties of opioid drugs?

A: The role of the Human OPRL1 Stable Cell Line-CHO in studying the addictive properties of opioid drugs is primarily to provide a platform that simulates human receptor activity for assessing drug affinity, agonist or antagonist activity, and drug-induced signaling pathways. This information is crucial for understanding how drugs affect opioid receptors in the brain and for designing drugs with lower potential for addiction.

How can the Human OPRL1 Stable Cell Line-CHO cell line be optimized to improve its application efficiency in drug development?

A: To optimize the Human OPRL1 Stable Cell Line-CHO for increased application efficiency in drug development, various strategies can be employed, such as improving cell culture conditions, optimizing gene expression levels, developing high-throughput screening methods, and implementing strict quality control processes. These measures help enhance the stability of the cell line, increase the accuracy and efficiency of drug screening, and thus accelerate the discovery and development of new drugs.

What role does the Human OPRL1 Stable Cell Line-CHO play in drug metabolism research?

A: The Human OPRL1 Stable Cell Line-CHO primarily serves as a model system in drug metabolism research to study the metabolic processes of drugs within the body. Using this cell line, scientists can simulate drug metabolism in the liver, assess the metabolic stability of drugs, and investigate the mechanisms of action of drug-metabolizing enzymes. This is crucial for predicting the pharmacokinetic properties of drugs and potential toxic reactions.

What are the potential applications of the Human OPRL1 Stable Cell Line-CHO in drug safety assessment?

A: The potential applications of the Human OPRL1 Stable Cell Line-CHO in drug safety assessment include evaluating the potential toxicity of drugs to opioid receptors, studying drug-induced cellular stress responses, and predicting potential side effects of drugs in the body. This cell line can help scientists identify potential safety risks at an early stage, allowing for the implementation of preventive measures during the drug development process.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *