Cat.No. : AD00203Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00203Z |
| Target Gene | MFN2 |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | MFN2 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Mitochondrial dynamics plays a crucial role in mitochondrial function. The mitofusin 2 (MFN2) gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion to maintain and operate the mitochondrial network. In addition, MFN2 is essential for mitophagy. Here, researchers investigated the role of MFN2 in mitochondrial fusion and mitophagy in angiotensin II-stimulated cardiomyocyte injury. The study showed that MFN2 expression gradually decreased with angiotensin II stimulation. MFN2 deficiency impaired mitochondrial quality, leading to aggravated angiotensin II-induced mitochondrial damage. MFN2 overexpression alleviated angiotensin II-induced ROS generation and increased apoptosis rate. In addition, MFN2 alleviated angiotensin II-induced MMP reduction. MFN2 promoted mitochondrial fusion, and MFN2 promoted Parkin translocation and phosphorylation, leading to mitophagy. The effects of MFN2 overexpression could be reversed by autophagy inhibitors.
To evaluate the effect of MFN2 overexpression on Ang II-induced cardiomyocyte injury, the researchers analyzed intracellular ROS generation, MMP, and apoptosis rate. Cardiomyocytes were divided into four groups: (1) Control+Ad-Control, cells were transfected with control adenovirus and not treated with Ang II; (2) Control+Ad-MFN2, cells were transfected with MFN2 adenovirus and not treated with Ang II; (3) Ang II+Ad-Control, cells were transfected with control adenovirus and treated with 1 μM Ang II for 24 h; (4) Ang II+Ad-MFN2, cells were transfected with MFN2 adenovirus and treated with 1 μM Ang II for 24 h. Compared with the Ang II+Ad-Control group, the ROS production in the Ang II+Ad-MFN2 group was significantly reduced (Figure 1A). In addition, after transfection with adenovirus overexpressing MFN2, JC-1 staining showed that overexpression of MFN2 alleviated the decrease of MMP caused by Ang II (Figure 1B). The decrease of MMP represents the early stage of apoptosis. In addition, TUNEL assay showed that overexpression of MFN2 significantly reduced AngII-induced cardiomyocyte apoptosis (Figure 1C). These results indicate that overexpression of MFN2 can prevent Ang II-induced damage, including oxidative stress, MMP, and cell apoptosis rate.
Figure 1. Overexpression of MFN2 prevented Ang II-induced cardiomyocyte injury. (Xiong W, et al., 2019)
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human MFN2 adenoviral particles exceeded our expectations. The viral prep was clean, and the gene expression was robust—great for mitochondrial research!
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
