Human MBD4 Knockdown Cell Line - Glioma

Name: Human MBD4 Knockdown Cell Line - Glioma
SKU: CSC-RK0229
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0229

Host Cell : Glioma Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-RK0229
Description	This cell line is engineered to stably express shRNA targeting human MBD4.
Target Gene	MBD4
Host Cell	Glioma
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Storage	Liquid nitrogen
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MBD4 methyl-CpG binding domain protein 4 [ Homo sapiens ]
Gene Symbol	MBD4
Synonyms	MBD4; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 4; MED1; G/T mismatch glycosylase; G/U mismatch glycosylase; methyl-CpG-binding protein MBD4; 3,N-ethenocytosine glycosylase; methyl-CpG-binding endonuclease 1; mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein; G/5-fluorouracil mismatch glycosylase with biphasic kinetics;
GeneID	8930
Uni ProtID	O95243
mRNA Refseq	BC011752
Chromosome Location	3q21.3
Function	DNA binding; catalytic activity; endodeoxyribonuclease activity; hydrolase activity; protein binding; sat
Pathway	Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; Cleavage of the damaged pyrimidine, organism-specific biosystem; DNA Repair, organism-specific biosystem;
MIM	603574

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