Cat.No. : CSC-RO0072 Host Cell: CHO-K1
| Cat. No. | CSC-RO0072 |
| Description | This cell line is a stably transfected cell line which expresses human inducible T-cell costimulator ligand (ICOSLG). |
| Background | ICOSLG protein is also known as CD275. This protein is encoded by the ICOSLG gene in human. |
| Gene | ICOSLG |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Target | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human ICOSLG Stable Cell Line-CHO (Chinese Hamster Ovary) offers several advantages for protein expression. Firstly, CHO cells are known for their ability to perform complex post-translational modifications, which are crucial for the proper folding and function of many therapeutic proteins. Secondly, the stable expression of ICOSLG in this cell line allows for the production of proteins with consistent glycosylation patterns, which can be critical for biopharmaceutical development. Thirdly, the Human ICOSLG Stable Cell Line-CHO may exhibit higher protein yields compared to other cell lines, which can be a significant factor in the cost-effectiveness of biomanufacturing.
A: The Human ICOSLG Stable Cell Line-CHO can be utilized for the production of monoclonal antibodies (mAbs) with human-like glycosylation patterns. This is particularly important because the glycosylation of mAbs can significantly influence their efficacy, stability, and immunogenicity. By expressing ICOSLG, the cell line can mimic the human glycosylation machinery, leading to mAbs with improved clinical properties.
A: Long-term culture of the Human ICOSLG Stable Cell Line-CHO can face challenges such as genetic drift, which may lead to changes in the expression of the ICOSLG gene or other cellular characteristics. Additionally, maintaining the stability of the cell line over time requires careful monitoring and selection strategies to ensure that the desired phenotype is preserved.
A: Scaling up protein production with the Human ICOSLG Stable Cell Line-CHO involves optimizing bioreactor conditions, such as nutrient supply, oxygen levels, and pH, to support cell growth and protein synthesis. Additionally, the process must be carefully monitored to ensure that the quality attributes of the protein, including glycosylation, remain consistent with those produced at smaller scales.
A: The Human ICOSLG Stable Cell Line-CHO can be used to produce proteins with personalized glycosylation profiles, which can be tailored to individual patients' needs. This capability is particularly relevant for the development of therapeutics that require specific glycosylation patterns for optimal efficacy and safety.
A: The use of the Human ICOSLG Stable Cell Line-CHO in clinical trials must comply with regulatory guidelines that ensure the safety, efficacy, and quality of the manufactured proteins. This includes demonstrating the stability of the cell line, the consistency of the protein product, and the absence of adventitious agents.
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