Human GLRA1 Stable Cell Line-HEK293

Name: Human GLRA1 Stable Cell Line-HEK293
SKU: CSC-RI0010
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0010

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0010
Description	This cell line is engineered to overexpress human GLRA1
Target Gene	GLRA1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuGLRA1
Alias	GLRA1, STHE, MGC138878, MGC138879
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GLRA1 glycine receptor, alpha 1 [ Homo sapiens ]
Gene Symbol	GLRA1
Synonyms	STHE; HKPX1
GeneID	2741
Uni ProtID	P23415
mRNA Refseq	NM_000171.3
Protein Refseq	NP_000162.2
Chromosome Location	5q32
Function	extracellular-glycine-gated chloride channel activity; contributes_to extracellular-glycine-gated chloride channel activity; extracellular-glycine-gated chloride channel activity; glycine binding; protein binding; taurine binding; transmitter-gated ion channel activity;
Pathway	Ion channel transport, organism-specific biosystem; Ligand-gated ion channel transport, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; Transmembrane transport of small molecules, organism-specific biosystem;
MIM	138491

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Background

Q & A

Customer Reviews

Inhibitory neurotransmission in the mammalian CNS is mainly mediated by the amino acids GABA and glycine. GABA receptors constitute major targets of widely used drugs such as barbiturates and benzodiazepines, whereas clinically applicable compounds that target GlyRs have yet to be identified. Glycine levels are highest in the brainstem and spinal cord where GlyRs are involved with the control of motor rhythm generation, the coordination of spinal responses and the processing of sensory signals. A disruption GlyR surface expression, or by reducing the ability of expressed GlyRs to conduct chloride ions, results in the rare neurological disorder, hyperekplexia. The disorder is characterized by an exaggerated response to unexpected stimuli which is followed by a temporary but complete muscular rigidity often resulting in an unprotected fall. Chronic injuries as a result of the falls are symptomatic of the disorder. A mutation in GLRA1 is responsible for some cases of stiff person syndrome.

Customer Q&As

What is the source of Human GLRA1 Stable Cell Line-HEK293, and how is it constructed?

A: Human GLRA1 Stable Cell Line-HEK293 is typically constructed by stably introducing the human GLRA1 gene into HEK293 cells. This can be achieved through techniques such as transfection or viral transduction.

What are the significant applications of Human GLRA1 Stable Cell Line-HEK293 in neurobiology research?

A: This cell line can be used in neurobiology research to study the function of the Glycine Receptor Alpha 1 subunit (GLRA1), neuronal signal transmission, drug screening, and research related to neurological disorders.

What are the common experimental protocols and techniques used when conducting electrophysiological experiments with Human GLRA1 Stable Cell Line-HEK293?

A: Electrophysiological experimental protocols may include membrane potential recordings, calcium imaging, and other techniques to investigate the function and activity of GLRA1 channels.

How is the drug effect on the Glycine Receptor Alpha 1 (GLRA1) evaluated in drug screening using Human GLRA1 Stable Cell Line-HEK293?

A: Evaluating the drug effect on GLRA1 typically involves testing at different drug concentrations to understand its impact on channel activity.

How is the stable expression of the human GLRA1 gene maintained in Human GLRA1 Stable Cell Line-HEK293?

A: Maintaining stable expression of the human GLRA1 gene may require the use of appropriate selective antibiotics and regular cell culture to ensure the stability of the GLRA1 gene in the cells.

How can Human GLRA1 Stable Cell Line-HEK293 simulate the pathophysiological processes of related neurological disorders in research?

A: This cell line can be used to simulate the pathophysiological processes of related neurological disorders by introducing relevant mutations or using GLRA1 genes from patients to study their effects on channel function.

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