Human GALT Knockout Cell Line-HeLa

Name: Human GALT Knockout Cell Line-HeLa
SKU: CSC-RT0716
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0716

Target Gene : GALT Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0716
Description	A stable cell line with a homozygous knockout of human GALT using CRISPR/Cas9.
Target Gene	GALT
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nitrogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GALT
GeneID	2592

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

Galactose-1-phosphate uridyltransferase (GALT) is a key enzyme in the Leloir pathway, responsible for galactose metabolism in organisms. The GALT enzyme itself is a homodimer, with each subunit consisting of approximately 379 amino acids. It facilitates the conversion of galactose-1-phosphate (Gal-1-P) and uridine diphosphoglucose (UDP-glucose) into UDP-galactose and glucose-1-phosphate. The proper function of this enzyme is essential for the efficient utilization and detoxification of galactose from the diet, primarily lactose in milk and dairy products.

Mutations in the GALT gene may result in insufficient or complete absence of enzyme activity, leading to a metabolic disorder known as classic galactosemia. This autosomal recessive disorder can have serious consequences if left untreated, including liver damage, cataracts, intellectual disability, and in severe cases, death. Infants with galactosemia often develop symptoms such as jaundice, vomiting, lethargy, and poor growth soon after drinking milk.

Galactosemia Research: Since the GALT (galactose-1-phosphate uridyltransferase) gene is essential for the normal metabolism of galactose, knocking out this gene in HeLa cells can mimic conditions such as classical galactosemia.

Drug Screening and Therapeutics: The GALT knockout cell line can be used for high-throughput drug screening to identify potential therapeutic compounds that can alleviate or correct the metabolic defects caused by GALT impairment. This is particularly useful for developing treatments for galactosemia and potentially related metabolic diseases.

Functional Genomics: Researchers can study how the loss of GALT affects cellular processes, gene expression, and metabolic networks. This helps understand the role of this gene beyond galactose metabolism and its impact on cell physiology.

Metabolic Flux Analysis: By using the GALT knockout HeLa cell line, scientists can perform flux analysis to track metabolic pathways that are altered due to the lack of a functional GALT.

Synthetic Biology and Metabolic Engineering: The GALT knockout cell line can be used in synthetic biology to engineer cells with customized metabolic pathways. By reintroducing and manipulating GALT in these cells, scientists can create customized metabolic models for a variety of applications, including bioproduction and anabolic circuits.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Time-Saving

The pre-engineered Human GALT Knockout Cell Line has saved us a significant amount of time and resources. Instead of developing our knockout models, we could instantly dive into experiments, which accelerated our research timeline exponentially.

United States

2024-03-02

Robust growth

We were pleasantly surprised by the robust growth and health of these knockout cell lines. They adapted well to our culture conditions and demonstrated excellent viability, which has been critical for our long-term experiments.

Germany

2024-07-12

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Human GALT Knockout Cell Line-HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Time-Saving

Robust growth

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