Human GABRA6 Stable Cell Line-HEK293

Name: Human GABRA6 Stable Cell Line-HEK293
SKU: CSC-RI0009

Cat.No. : CSC-RI0009 Host Cell: HEK293

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Cat. No.	CSC-RI0009
Description	This cell line is engineered to overexpress human GABRA6
Gene	GABRA6
Gene Species	Homo sapiens (Human)
Alias	GABRA6, MGC116903, MGC116904
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Gamma-aminobutyric acid (GABA)-gated ion channels are widely distributed in the mammalian brain and are major mediators of inhibitory synaptic transmission. A typical GABA ion channel has a pentameric structure consisting of 5 protein subunits, often alpha, beta and gamma or delta, combining to form a central ion conducting pore across the cell membrane. In humans there are six genes that encode alpha subunits, three that encode beta, three that encode gamma, and an additional seven genes that encode other subunits whose function is less-well understood than the alpha, beta and gamma subunits. GABA ion channels open and close in response to secretion of GABA from presynaptic terminals. GABAA alpha6 channels are typically comprised of alpha6, beta2 or beta3, and gamma2 subunits and this combination is preferentially expressed in the mammalian cerebellum in granule cells. GABAA alpha6-containing receptors are located synaptically and comprise<5% of="" all="" gabaa="" receptors="" located="" in="" the="" brain.="" these="" receptors="" are="" insensitive="" to="">

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