Cat.No. : CSC-RG0059 Host Cell: CHO-K1
| Cat. No. | CSC-RG0059 |
| Background | Free fatty acid receptor 3 (FFA3) is a G-protein coupled receptor that in humans is encoded by the FFAR3 gene. Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate >acetate >formate. |
| Gene | FFAR3 |
| Gene Species | Homo sapiens (Human) |
| Alias | FFAR3, GPR41, FFA3R |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human FFAR3 Stable Cell Line-CHO (Chinese Hamster Ovary) is engineered to express the human FFAR3 receptor at a high level, which allows for the investigation of FFAR3-mediated cellular responses. This cell line is particularly useful for studying the downstream signaling of FFAR3, such as its role in glucose metabolism and inflammation, due to its ability to mimic human cell behavior.
A: While primary human cells are derived directly from human tissues and thus represent the native context of FFAR3 function, the Human FFAR3 Stable Cell Line-CHO is a transformed cell line that has been genetically modified to stably express the human FFAR3 receptor. This engineered cell line provides a controlled environment for studying FFAR3, but it may not fully replicate the complexity of FFAR3 function in primary cells.
A: The Human FFAR3 Stable Cell Line-CHO offers several advantages for high-throughput screening. It provides a consistent and reproducible model for evaluating the efficacy and safety of compounds that modulate FFAR3 activity. Additionally, the use of a cell line allows for the automation of assays, which increases the efficiency and throughput of compound testing.
A: By providing a model system where FFAR3 can be manipulated and its effects monitored, the Human FFAR3 Stable Cell Line-CHO aids in elucidating the receptor's role in metabolic processes. This can lead to a better understanding of how FFAR3 contributes to diseases like diabetes and obesity, which in turn can inform the development of new therapeutic strategies.
A: By studying the response of the Human FFAR3 Stable Cell Line-CHO to various compounds, researchers can identify potential therapeutic agents that target FFAR3. This information can then be used to develop personalized treatment plans based on an individual's genetic makeup and the specific characteristics of their FFAR3 receptor.
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