Human FFAR3 Stable Cell Line-CHO-K1

Name: Human FFAR3 Stable Cell Line-CHO-K1
SKU: CSC-RG0059
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0059

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0059
Background	Free fatty acid receptor 3 (FFA3) is a G-protein coupled receptor that in humans is encoded by the FFAR3 gene. Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate >acetate >formate.
Target Gene	FFAR3
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuFFAR3
Alias	FFAR3, GPR41, FFA3R
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FFAR3 free fatty acid receptor 3 [ Homo sapiens ]
Gene Symbol	FFAR3
Synonyms	FFAR3; free fatty acid receptor 3; G protein coupled receptor 41 , GPR41; FFA3R; G protein-coupled receptor 41; G-protein coupled receptor 41; GPR41;
GeneID	2865
Uni ProtID	O14843
mRNA Refseq	BC035657
Chromosome Location	19q13.1
Function	G-protein coupled receptor activity; lipid binding; receptor activity; signal transducer activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Free fatty acid receptors, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
MIM	603821

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Customer Q&As

What are the key molecular characteristics of the Human FFAR3 Stable Cell Line-CHO that make it suitable for studying FFAR3 signaling pathways?

A: The Human FFAR3 Stable Cell Line-CHO (Chinese Hamster Ovary) is engineered to express the human FFAR3 receptor at a high level, which allows for the investigation of FFAR3-mediated cellular responses. This cell line is particularly useful for studying the downstream signaling of FFAR3, such as its role in glucose metabolism and inflammation, due to its ability to mimic human cell behavior.

How does the Human FFAR3 Stable Cell Line-CHO differ from primary human cells in terms of FFAR3 function?

A: While primary human cells are derived directly from human tissues and thus represent the native context of FFAR3 function, the Human FFAR3 Stable Cell Line-CHO is a transformed cell line that has been genetically modified to stably express the human FFAR3 receptor. This engineered cell line provides a controlled environment for studying FFAR3, but it may not fully replicate the complexity of FFAR3 function in primary cells.

What are the advantages of using the Human FFAR3 Stable Cell Line-CHO for high-throughput screening of compounds targeting FFAR3?

A: The Human FFAR3 Stable Cell Line-CHO offers several advantages for high-throughput screening. It provides a consistent and reproducible model for evaluating the efficacy and safety of compounds that modulate FFAR3 activity. Additionally, the use of a cell line allows for the automation of assays, which increases the efficiency and throughput of compound testing.

How does the Human FFAR3 Stable Cell Line-CHO contribute to the understanding of FFAR3's role in metabolic diseases?

A: By providing a model system where FFAR3 can be manipulated and its effects monitored, the Human FFAR3 Stable Cell Line-CHO aids in elucidating the receptor's role in metabolic processes. This can lead to a better understanding of how FFAR3 contributes to diseases like diabetes and obesity, which in turn can inform the development of new therapeutic strategies.

How can the Human FFAR3 Stable Cell Line-CHO be utilized in the development of personalized medicine approaches for FFAR3-related conditions?

A: By studying the response of the Human FFAR3 Stable Cell Line-CHO to various compounds, researchers can identify potential therapeutic agents that target FFAR3. This information can then be used to develop personalized treatment plans based on an individual's genetic makeup and the specific characteristics of their FFAR3 receptor.

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