Human FFAR2 Stable Cell Line-CHO-K1

Name: Human FFAR2 Stable Cell Line-CHO-K1
SKU: CSC-RG0064
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0064

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0064
Background	This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels.
Target Gene	FFAR2
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuFFAR2
Alias	FFAR2, FFA2R, GPR43
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FFAR2 free fatty acid receptor 2 [ Homo sapiens ]
Gene Symbol	FFAR2
Synonyms	FFA2R; GPR43
Gene ID	2867
Uni Prot ID	O15552
m RNA Refseq	BC096198
Chromosome Location	19q13.1
Function	G-protein coupled receptor activity;lipid binding;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem;Free fatty acid receptors, organism-specific biosystem;G alpha (q) signalling events, organism-specific biosystem;GPCR downstream signaling, organism-specific biosystem;GPCR ligand binding, organism-specific biosystem;GPCRs, Class A Rhodopsin-like, organism-specific biosystem;Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem;
MIM	603823

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Customer Reviews

Customer Q&As

What advantages does the Human FFAR2 Stable Cell Line-CHO offer in studying the FFAR2 signaling pathway?

A: This cell line provides a stable platform for studying the FFAR2 signaling pathway. Since FFAR2 plays a role in various physiological processes, including energy metabolism and inflammatory responses, being able to study these processes in a controlled environment is crucial for understanding its role in disease. The Human FFAR2 Stable Cell Line-CHO allows scientists to precisely control FFAR2 expression through genetic engineering, thereby revealing its specific role in cellular signaling.

How is the Human FFAR2 Stable Cell Line-CHO applied in studying FFAR2-mediated inflammatory responses?

A: In studying FFAR2-mediated inflammatory responses, the Human FFAR2 Stable Cell Line-CHO can be used to simulate cellular behavior under inflammatory conditions. By adding inflammatory mediators or mimicking an inflammatory environment, researchers can observe changes in FFAR2 activity during the inflammatory process and how it regulates the expression of inflammation-related genes. This cell line helps to elucidate the mechanism of action of FFAR2 in inflammatory diseases and provides a theoretical basis for the development of anti-inflammatory drugs.

How can the Human FFAR2 Stable Cell Line-CHO be optimized to improve its efficiency in drug screening?

A: To improve the efficiency of the Human FFAR2 Stable Cell Line-CHO in drug screening, various strategies can be employed. For instance, optimizing culture conditions to enhance cell growth and stability, or using genetic engineering techniques to increase the expression level of FFAR2. Additionally, developing more sensitive assays to evaluate the impact of drugs on FFAR2 activity, and utilizing high-throughput automation equipment to accelerate the screening process can also be beneficial. These optimization measures help to increase the throughput and accuracy of screening, thus accelerating drug discovery.

What is the application prospect of the Human FFAR2 Stable Cell Line-CHO in studying the association between FFAR2 and metabolic diseases?

A: The Human FFAR2 Stable Cell Line-CHO has a broad application prospect in studying the association between FFAR2 and metabolic diseases. Given the important role of FFAR2 in regulating fat metabolism and insulin sensitivity, this cell line can be used to investigate the mechanisms of these diseases. By screening compounds that can modulate FFAR2 activity, scientists can discover new therapeutic targets and provide a foundation for developing new treatments for metabolic diseases such as diabetes and obesity.

What is the role of the Human FFAR2 Stable Cell Line-CHO in drug screening?

A: The Human FFAR2 Stable Cell Line-CHO (human FFAR2 stably expressed in Chinese hamster ovary cells) plays a pivotal role in drug screening by allowing researchers to simulate the function of FFAR2 (Free Fatty Acid Receptor 2) in vitro. This cell line can be used for high-throughput screening (HTS) and drug discovery to identify and optimize small molecules or biologics that may affect FFAR2 activity. In this way, scientists can develop treatments for diseases related to FFAR2, such as metabolic disorders, inflammation, and cardiovascular diseases.

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