Cat.No. : AD00166Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00166Z |
| Target Gene | FABP4 |
| Product Type | Adenoviral particle |
| Insert | FABP4 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | FABP4 fatty acid binding protein 4, adipocyte [ Homo sapiens ] |
| Gene Symbol | FABP4 |
| Synonyms | aP2; ALBP; AFABP; A-FABP |
| Gene Description | fatty acid binding protein 4, adipocyte |
| Gene ID | 2167 |
| UniProt ID | E7DVW4 |
| mRNA Refseq | NM_001442.2 |
| Protein Refseq | NP_001433.1 |
| Chromosome Location | 8q21 |
| Function | fatty acid binding; transporter activity; |
| Pathway | Developmental Biology, organism-specific biosystem; Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem; |
| MIM | 600434 |
Colon cancer metastasis carries a poor prognosis, but its mechanisms, especially those related to adipocytes, remain unclear. Fatty acid binding proteins (FABPs) are a class of homologous small cytoplasmic proteins that bind and transport hydrophobic fatty acids. FABP4, a member of this family, is highly expressed in adipose tissue and macrophages. Here, researchers observed higher lipid accumulation and stronger FABP4 transcription in colon cancer tissues. After incubation with adipose tissue extracts and overexpression of FABP4, colon cancer cells showed enhanced lipid accumulation. In functional experiments, co-culture with adipose tissue extracts significantly enhanced the invasion and migration of colon cancer cells, as well as energy and lipid metabolism, all of which were reversed by FABP4 inhibitors. In addition, the metastasis of colon cancer cells overexpressing FABP4 was also significantly enhanced in both in vivo and in vitro experiments. In terms of mechanism, bioinformatics analysis showed that FABP4 was enriched in 11 pathways related to metabolic processes in overexpressing cells. Finally, FABP4 overexpression promotes EMT progression in colon cancer, as evidenced by upregulation of Snail, MMP-2, and MMP-9, downregulation of E-cadherin, and increased expression of p-Akt. These studies indicate that FABP4 overexpression can increase fatty acid transport, enhance energy and lipid metabolism, activate the AKT pathway and EMT, and promote migration and invasion of colon cancer cells.
To further explore the mechanism of action of FABP4 in colon cancer metastasis, FABP4 recombinant adenovirus was used to confirm that FABP4 was involved in the progression of colon cancer. In HCT-8 cells, the number of transmembrane cells in the FABP4 overexpression group was 1.70±0.03 times that of the control group (Figure 1d). The width of the "scratch" in the FABP4 overexpression group after 24 hours of scratching was 76% of that in the control group (Figure 1e). However, there was no change in the proliferation of cancer cells (Figure 1f). Similar results were observed in HCT-116 cells. Overall, these results indicate that FABP4 enhances the invasion and migration of colon cancer cells but does not change their proliferation.
Figure 1. FABP4 enhanced the invasion and migration, but did not change the proliferation of colon cancer cells. (Tian W, et al., 2020)
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