Cat.No. : CSC-RG0066 Host Cell: CHO-K1
| Cat. No. | CSC-RG0066 |
| Background | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. |
| Gene | CHRM2 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM2, HM2, FLJ43243, MGC120006, MGC120007 |
| Host Cell | CHO-K1 |
| Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: In drug screening, the Human CHRM2 Stable Cell Line-CHO has significant advantages. Firstly, CHO cell lines grow rapidly and are easy to genetically manipulate, allowing for the quick production of large quantities of cells for experiments. Secondly, the stable expression of the CHRM2 receptor enables the cell line to specifically respond to cholinergic signals, which is crucial for studying and developing new drugs targeting the cholinergic system. Additionally, the xenogenic expression capability of CHO cell lines allows them to mimic the human cellular environment, thereby improving the predictive accuracy of drug screening.
A: The Human CHRM2 Stable Cell Line-CHO plays a significant role in the study of neurodegenerative diseases. Since the CHRM2 receptor is closely associated with cognitive functions and memory formation in the brain, this cell line can mimic the activity of these receptors in neurons, helping researchers understand the dysfunction of the cholinergic system in these diseases. By conducting drug screening and toxicity tests in these cells, scientists can identify potential therapeutic targets and develop new treatment approaches.
A: To perform dose-response curve analysis of drugs using the Human CHRM2 Stable Cell Line-CHO cell line, a series of drug treatments at varying concentrations must be designed. Then, the cells are exposed to these concentrations to assess the impact on CHRM2 receptor activity. By measuring the degree of receptor activation or inhibition, a relationship between drug concentration and biological effect can be plotted. This analysis helps determine the effective concentration range and maximum effect of the drug, providing data support for further drug development and optimization.
A: To assess the affinity and selectivity of drugs to the CHRM2 receptor, radioligand binding assays can be used. A suitable radiolabeled ligand, such as 3H-QNB, is incubated with the cell line, and the competition for receptor binding is observed by varying the drug concentration. Affinity can be assessed by calculating the drug's half-maximal inhibitory concentration (IC50), while selectivity is determined by comparing the binding affinity to different receptor subtypes. This method provides detailed information about the drug's interaction with the CHRM2 receptor, guiding drug design.
A: The applications of the Human CHRM2 Stable Cell Line-CHO cell line in drug metabolism research mainly focus on evaluating the metabolic stability of drugs in the body and the formation of potential metabolites. By adding drugs to these cells, the metabolic rate and formation of metabolites can be monitored. Additionally, the cell line can be used to study the interaction between drugs and metabolic enzymes, such as the CYP450 enzyme family, which is crucial for predicting the pharmacokinetic properties of drugs and potential drug-drug interactions.
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