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Cat.No. : CSC-RT0077
Host Cell: HCT116 Target Gene: CDK2/TP53
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0077 |
| Description | HCT116 -CDK2/TP53 (-/-,-/-) is a cell line with a double homozygous knockout of human CDK2 and TP53 |
| Target Gene | CDK2/TP53 |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The knockdown efficiency of Human CDK2/TP53 Knockout Cell Line-HCT116 is unmatched by any other reagent I have used before.
United States
04/01/2023
Using Human CDK2/TP53 Knockout Cell Line-HCT116 has been a highly competent helper for my research. I am incredibly impressed with its efficiency and reliability.
United States
08/23/2020
Human CDK2/TP53 Knockout Cell Line-HCT116 is a must-have for any researcher looking to achieve efficient and effective gene editing.
United States
05/12/2021
I have recommended Human CDK2/TP53 Knockout Cell Line-HCT116 to all of my colleagues. It has made such a difference in my research.
United States
08/15/2021
I have used several gene knockout reagents in the course of my previous experiments, but Human CDK2/TP53 Knockout Cell Line-HCT116 is definitely the best. It consistently produces high-quality results and is very user-friendly.
Germany
10/09/2021
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