Cat.No. : CSC-RR0120 Host Cell: HCT116
| Cat. No. | CSC-RR0120 |
| Description | The HCT-116 line was isolated from a male with colonic carcinoma. The cells are positive for keratin by immunoperoxidase staining and are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.The GFP Stable Cell Line-HCT-116 constitutively expresses GFP. |
| Background | The GFP Stable Cell Line-HCT-116 originates from the development of stable cell lines expressing Green Fluorescent Protein (GFP) within the HCT-116 cell line background. This cell line has been instrumental in elucidating various cellular processes and pathways due to its ability to visualize protein localization |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Colorectal cancer (CRC) is a prevalent cancer worldwide, but the role and mechanisms of ABL1 (c-Abl), a non-receptor tyrosine kinase, in CRC are not fully understood. Researchers investigated the role of ABL1 in CRC using GFP Stable Cell Line-HCT-116. Through analysis of patient samples, CRC cell lines, and mouse models, they found that ABL1 expression was associated with CRC progression. Depletion of ABL1 inhibited CRC cell proliferation and induced apoptosis in vitro and in vivo. Ingenuity pathway analysis revealed ABL1's involvement in regulating TGF-β1 via the IRS1/PI3K/AKT pathway in CRC. These findings highlight ABL1 as a potential therapeutic target for CRC treatment.
Figure 1. Researchers used GFP Stable Cell Line-HCT-116 to study TGF-β1's role in PI3K/AKT pathway regulation. Knockdown of TGF-β1 decreased GFP fluorescence, confirming successful targeting. Western blot analysis revealed suppressed pathway activity post-TGF-β1 knockdown, offering insights into cancer signaling. Conversely, PPP3CA knockdown showed no significant effects on GFP fluorescence or pathway activity. This model facilitates research on TGF-β1-mediated signaling in cancer cells. (Liu Y, et al., 2020)
A: HCT-116 cells were chosen for establishing the GFP stable cell line due to their relevance to colorectal cancer research and their well-characterized genetic background. Additionally, HCT-116 cells exhibit high transfection efficiency, making them suitable for stable expression studies.
A: The stability and expression level of GFP in the HCT-116 stable cell line was ensured and maintained through stable transfection techniques, antibiotic selection, and validation of expression using fluorescence microscopy or flow cytometry. Regular subculture and monitoring of fluorescence intensity were employed to sustain stable expression levels.
A: Functional characterization of GFP in the HCT-116 stable cell line involved assessing its responsiveness to regulatory elements by analyzing changes in fluorescence intensity in response to stimuli. Additionally, GFP's involvement in cellular processes such as protein localization, organelle tracking, or cell cycle dynamics was investigated using live-cell imaging and fluorescence microscopy.
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An invaluable tool! The GFP Stable Cell Line has significantly enhanced my research capabilities, offering a reliable platform for studying colorectal cancer biology and evaluating therapeutic strategies in vitro.
Streamlining research processes! Its stable GFP expression simplifies experimental workflows, allowing for efficient data collection and analysis, and accelerating discoveries in cancer research.
This cell line surpasses expectations, providing bright and uniform GFP fluorescence, facilitating accurate quantification and imaging of tumor cells in vitro.
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