Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0105
Host Cell : BT474 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0105 |
| Description | The BT-474 cell line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast of a 60-year old female. The GFP Stable Cell Line-BT474 constitutively expresses GFP. |
| Host Cell | BT474 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter Type | Fluorescent protein |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Recommended Medium | Inquiry for instruction of culturing |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
BT-474 is a human breast cancer cell line established from primary tumors of breast cancer patients. The BT474 cell line has several properties that make it a useful tool in cancer research. It can grow in culture and exhibit many of the same properties as breast cancer cells found in patients. Researchers used the BT-474 cell line to study the molecular mechanisms of breast cancer, including genes and pathways involved in cancer cell growth, invasion and metastasis. The BT-474 cell line is also used to test the efficacy of various cancer treatments, including chemotherapy, radiation therapy and targeted therapy.
The GFP stable cell line-BT474 is generated by transforming BT474 cells with a plasmid carrying the GFP gene, resulting in stable integration of the gene into the genome of the BT474 cells. The GFP stable cell line-BT474 enables researchers to monitor the in vitro and in vivo behavior of BT474 subtype breast cancer cells under a variety of experimental conditions. GFP fluorescence provides a real-time, non-invasive indication of cell status, allowing for continuous monitoring and analysis. It can be used to study proliferation, migration, invasion and protein localization as well as other cellular functions in living organisms or populations of living cells in real time. Therefore, the GFP stable cell line-BT474 is a valuable tool in various areas of biomedical research, including cancer biology, drug discovery and development, cell biology, and preclinical research.
GFP stable cell line-BT474 has a wide range of applications in the field of biological research, especially in the fields of cancer research, cell biology and gene expression research. The following are potential uses for this cell line:
Drug testing: This cell line can be used to screen and test the efficacy and toxicity of potential anti-cancer drugs. The survival rate of GFP-expressing cells can be a direct indicator of drug toxicity.
Tumor growth and metastasis studies: BT474 cells are widely used in breast cancer research because they are derived from human breast ductal carcinoma. They are often used to study tumor growth and metastasis.
Gene expression studies: This GFP cell line can be used to study gene expression and regulation. By inserting a specific gene into the GFP sequence, researchers can track the expression of that gene by observing GFP fluorescence.
Protein-protein interaction studies: These cells can fluoresce when proteins bind to GFP, making them valuable in studies of protein-protein interactions.
Cell biology research: GFP stable cell line-BT474 can be used to study various cellular processes, including cell division, differentiation and apoptosis, by observing the fluorescence of GFP.
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We have been using GFP Stable Cell Line-BT474 for several months with excellent results. The stable and robust GFP expression makes it an excellent tool for our live cell imaging experiments.
Our lab needed a stable cell line with reliable GFP expression for drug screening experiments, and GFP Stable Cell Line-BT474 performed beyond expectations.
The GFP Stable Cell Line-BT474 has been a fantastic addition to our lab's toolkit. The green fluorescence is bright and stable, facilitating effortless visualization under the microscope.
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