Cat.No. : CSC-RR0103 Host Cell: 4T1
| Cat. No. | CSC-RR0103 |
| Description | 4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment. The GFP Stable Cell Line-4T1(Mouse) constitutively expresses GFP. |
| Host Cell | 4T1 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Metastasis is the process by which cancer cells spread from their primary location to distant organs, and it is a key challenge in cancer treatment. During this process, circulating tumor cells (CTCs) interact with the endothelium, which plays a critical role in their arrest and subsequent colonization at metastatic sites. Researchers have explored this interaction to understand how CTCs either extravasate into tissues or remain in the bloodstream, where they may proliferate. A recent study used GFP-labeled 4T1 breast cancer cells in a mouse model to analyze these early stages of metastasis at the cellular level. By tracking CTCs and endothelial cells, the study revealed that endothelial-derived Wnt factors influence whether CTCs follow a route of extravasation and latency or remain in circulation, proliferating.
Figure 1. The researchers used FACS to purify extravascular and intravascular tumor cells, utilizing 4T1-GFP cells to track metastasis. They quantified cell proliferation in lung tissues post-injection to study tumor cell dormancy and extravasation. (Jakab M, et al., 2024)
Creative Biogene's GFP Stable Cell Line-4T1 (Mouse) offers a similar potential for metastasis research. This cell line expresses GFP, enabling real-time tracking in vivo and at a single-cell level, making it ideal for studying tumor cell dissemination, tumor-endothelial interactions, and the mechanisms of metastatic dormancy or progression.
A: The GFP expression allows for easy tracking of 4T1 cells in vivo, facilitating studies on the metastatic spread of breast cancer to distant organs in mouse models. This is critical for understanding the cellular and molecular mechanisms of metastasis.
A: Anti-metastatic compounds can be administered to mice bearing GFP-labeled 4T1 tumors, and the progression of metastasis can be monitored by detecting GFP expression in tissues, providing a direct measure of compound efficacy.
A: GFP labeling simplifies the visualization and quantification of 4T1 cells in invasion and migration assays, such as transwell and wound-healing assays, allowing for real-time observation and analysis of cell movement.
A: Yes, the fluorescent nature of GFP allows for live imaging of tumor cells in culture or in live animals, enabling dynamic studies of tumor growth, cell-cell interactions, and the response to the tumor microenvironment.
A: The GFP expression allows for the tracking of targeted delivery systems to the tumor site. By conjugating therapeutic agents with fluorescent markers and co-localizing with GFP, researchers can assess the targeting efficiency and therapeutic delivery to 4T1 tumors.
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This cell line exhibits robust GFP expression, which allows for straightforward visualization of the cells in vivo or in vitro, facilitating various experimental applications.
Being a model for breast cancer, GFP Stable Cell Line-4T1 mimics the metastatic behavior of tumors, enabling studies on cancer metastasis and testing of anti-metastatic agents.
The GFP Stable Cell Line-4T1 is compatible with the mouse immune system, which is crucial for studies involving immune response to cancer or other pathologies.
GFP fluorescence in the GFP Stable Cell Line-4T1 simplifies the monitoring of tumor growth and spread in live animals, providing a non-invasive method to study disease progression.
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