Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-DC011243 | Panoply™ Human PAF1 Knockdown Stable Cell Line | Inquiry |
| CSC-DC011609 | Panoply™ Human PEX2 Knockdown Stable Cell Line | Inquiry |
| CSC-SC011243 | Panoply™ Human PAF1 Over-expressing Stable Cell Line | Inquiry |
| CSC-SC011609 | Panoply™ Human PEX2 Over-expressing Stable Cell Line | Inquiry |
| CSC-RT2471 | Human PAF1 Knockout Cell Line-HEK293T | Inquiry |
| Cat.No. | Product Name | Price |
|---|---|---|
| AD11751Z | Human PAF1 adenoviral particles | Inquiry |
| AD12107Z | Human Pex2 adenoviral particles | Inquiry |
| LV20877L | human PAF1 (NM_019088) lentivirus particles | Inquiry |
| LV21504L | human PEX2 (NM_001079867) lentivirus particles | Inquiry |
| Cat.No. | Product Name | Price |
|---|---|---|
| SHW000774 | shRNA set against Chicken PEX2 (NM_001008454) | Inquiry |
| SHH366044 | shRNA set against Rat Olr820 (NM_001000974.1) | Inquiry |
| SHH369988 | shRNA set against Human PAF1 (NM_019088.3) | Inquiry |
| SHH369992 | shRNA set against Mouse PAF1 (NM_019458.3) | Inquiry |
| SHH369996 | shRNA set against Rat PAF1 (NM_001024898.1) | Inquiry |
| SHH373744 | shRNA set against Human Pex2 (NM_000318.2) | Inquiry |
| SHH373748 | shRNA set against Mouse Pex2 (NM_028730.5) | Inquiry |
| SHH373752 | shRNA set against Rat Pex2 (NM_001106618.2) | Inquiry |
| SHH387324 | shRNA set against Mouse PXMP3 (NM_008994.4) | Inquiry |
| SHH387328 | shRNA set against Rat PXMP3 (NM_017234.1) | Inquiry |
| SHR077946 | shRNA set against Rat Paf1(NM_001024898.1) | Inquiry |
| SHR096378 | shRNA set against Rat Pex2(NM_173152.1) | Inquiry |
| SHR096438 | shRNA set against Human PXMP3(NM_000318.2) | Inquiry |
| SHW008932 | shRNA set against Danio rerio PAF1 (NM_001024453) | Inquiry |
| Cat.No. | Product Name | Price |
|---|---|---|
| SKO0650 | PAF1 Validated sgRNA vector | Inquiry |
| MiUTR1R-06228 | PEX2 miRNA 3'UTR clone | Inquiry |
| MiUTR1R-05599 | PAF1 miRNA 3'UTR clone | Inquiry |
| MiUTR1R-05433 | OLR820 miRNA 3'UTR clone | Inquiry |
| MiUTR1M-08865 | PAF1 miRNA 3'UTR clone | Inquiry |
| MiUTR1H-08515 | PEX2 miRNA 3'UTR clone | Inquiry |
| CDFR011284 | Rat Pex2 cDNA Clone(NM_017234.1) | Inquiry |
| CDFR003732 | Rat Paf1 cDNA Clone(NM_001024898.1) | Inquiry |
| CDFR000946 | Rat Olr820 cDNA Clone(NM_001000974.1) | Inquiry |
| CDFH014095 | Human PEX2 cDNA Clone(NM_001172087.1) | Inquiry |
| CDFH014094 | Human PEX2 cDNA Clone(NM_001172086.1) | Inquiry |
| CDFH014093 | Human PEX2 cDNA Clone(NM_001079867.1) | Inquiry |
| MiUTR3H-11275 | PAF1 miRNA 3'UTR clone | Inquiry |
| CDCS419216 | Human PEX2 ORF Clone (BC005375) | Inquiry |
| CDCR378298 | Rat Pex2 ORF Clone(NM_017234.1) | Inquiry |
| CDCR370755 | Rat Paf1 ORF Clone(NM_001024898.1) | Inquiry |
| CDCR356409 | Human PEX2 ORF Clone(NM_001172087.1) | Inquiry |
| CDCR356408 | Human PEX2 ORF Clone(NM_001172086.1) | Inquiry |
| CDCR345474 | Human PEX2 ORF Clone(NM_001079867.1) | Inquiry |
| CDCR253857 | Mouse Paf1 ORF Clone(NM_019458.3) | Inquiry |
| CDCR246518 | Mouse Pex2 ORF Clone(NM_008994.4) | Inquiry |
| CDCR239685 | Mouse Pex2 ORF Clone(NM_001163305.2) | Inquiry |
| CDCR239679 | Mouse Pex2 ORF Clone(NM_001163302.2) | Inquiry |
| CDCR239677 | Mouse Pex2 ORF Clone(NM_001163301.2) | Inquiry |
| CDCG000253 | Human PEX2 ORF clone(NM_000318.2) | Inquiry |
| CDCB190819 | Rabbit PEX2 ORF clone (XM_008255682.1) | Inquiry |
| CDCB183428 | Rabbit PAF1 ORF clone (XM_002711452.2) | Inquiry |
| CDCB170407 | Danio rerio PAF1 ORF Clone (NM_001024453) | Inquiry |
| CDCB162249 | Chicken PEX2 ORF Clone (NM_001008454) | Inquiry |
| CDCR368033 | Rat Olr820 ORF Clone(NM_001000974.1) | Inquiry |
| CDCB158822 | Human PEX2 ORF clone (BC005375) | Inquiry |
PAF1 works as promoter-proximal pausing molecular regulator by RNA polymerase II
Especially in environmental and developmental cues responding genes, control of promoter-proximal pausing and RNA polymerase II (Pol II) releasing is recognized as the mechanism for regulating gene expression in metazoans. Pol-II-associated factor 1 (PAF1) was identified with the evolutionarily conserved function in metazoans for the promoter-proximal pausing regulation. PAF1 levels reduction can cause an increased release of paused Pol ii into gene bodies at thousands of genes, more than this, depletion of PAF1 gives the consequence of increased nascent and mature transcripts and increased levels of phosphorylation of Poi II’s C-terminal domain on serine 2 (Ser2P). All those changes may be accounted for Ser2P kinase super elongation complex (SEC) recruitment affected the release of paused Pol II into productive elongation increasing.
PD2/PAF1 at the crossroads of the cancer network
In mRNA stability and quality control, the core subunit of the human PAF1 complex (PAF1C), Pancreatic differentiation 2 (PD2)/RNA polymerase II-associated factor (PAF1), is responsible for promoter-proximal pausing of RNA polymerase II as well as transcription elongation and mRNA processing and coordinates events regulation. For its role in posttranslational histone covalent modifications as well as expression of critical genes of the cell-cycle machinery, PD2/PAF is recognized as an integral part of its transcription-regulatory function. The part of PAF1C, PD2/PAF1 alone can provide distinct roles in the maintenance of self-renewal of embryonic stem cells and cancer stem cells. So normal cellular functions affected by the malfunction or altered abundance of PD2/PAF1 can lead to disease states. Up-regulation of PD2/PAF1 can be seen in poorly differentiated pancreatic cancer cells, and is capable of neoplastic transformation when ectopically expressed in mouse fibroblast cells. In the same way, up-regulation of PD2/PAF1 is founded in pancreatic and ovarian cancer stem cells. There is a concise description that the multifaceted roles of PD2/PAF1 are oncogenic transformation associated and implicate PD2/PAF1 as an attractive target for therapeutic development to combat malignancy.
Figure 1. PAF1 works as a molecular regulator of promoter-proximal pausing by RNA polymerase II. (Fei Xavier Chen, et al. 2015)
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