Creative Biogene is a professional biotechnology company in CRISPR editing field. We have developed a mature CRISPR platform for editing microorganisms’ genome with a wide range of microbe species. As a pioneer in this field, Creative Biogene could provide you the best service with very short turnaround time and the most competitive price.
Creative Biogene could provide you microbe genome editing services based on our CRISPR platform, which including gene knockout, site-directed mutation, gene knock-in, and so on.
Found in bacteria and archaebacteria, the regular cluster-interval short palindromic repeat (CRISPR) can effectively resist the invasion and insertion of phage and various gene elements at the gene level. CRISPR-Associate (Cas) protein is a kind of RNA-mediated double-stranded DNA endonuclease which own the ability to cleave exogenous DNA. When the conserved spacer proto-adjacent motif (PAM) is present in the invading DNA sequence, the target DNA sequence will be cleaved in the spacer sequence upstream (4-5nt) of the PAM. Because CRISPR-Cas9 system requires only one single Cas9 endonuclease to complete the reaction, and the system is flexible, simple, specific and low cytotoxicity. It has become a popular genome editing technology and widely used in genome engineering.
Fig.1 The schematic of CRISPR Mediated Microbe Genome Editing (Wang, et al. 2016)
Creative Biogene has already developed a mature system for a broad range of microorganisms, including Escherichia coli, Salmonella, Streptomyces (including the model Streptomyces and common Streptomyces), solventogenic Clostridium (and other common Clostridium), and Saccharomyces cerevisiae, and so on.
The advantages of CRISPR based microbe genome editing in Creative Biogene:
Fast turnaround time
Scarless genome editing
Easy for the following researches
Strong background in developing CRISPR method for new microorganisms
With years of experience in CRISPR based genome editing field, Creative Biogene could provide the most excellent service for microorganisms’ genome editing. Our talent experts are dedicated to applying CRISPR system to edit your specific microbe with the greatest chance to succeed. Meanwhile, based on the commitment of prompt communication and on-time reporting, our staffs will ensure a high-efficiency service to meet the strict project timelines.
If you have any special requirements in our CRISPR based microbe genome editing service, please feel free to contact us at firstname.lastname@example.org or 1-631-626-9181. We are looking forward to working together with your attractive projects.
1. Yi W., Zhong-Tian, Z., Seung-Oh, S., Patrick, L., Ting, L., Yong-Su, J., Hans, P. B. (2016) 'Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable "Clean" Mutant Selection in Clostridium beijerinckii as an Example'. ACS Synth Biol., 5(7), 721-32