Y1H cDNA Library Screening Services

Fig.1 Outline of variations and considerations in Y1H assays. [1]

Yeast one-hybrid screening is a versatile and efficient method to rapidly identify heterologous transcription factors (or other DNA-binding proteins) that can interact with a specific regulatory DNA sequence of interest (the bait sequence). The one-hybrid screen is derived from the two-hybrid concept. In the two-hybrid technique, the interaction between two proteins (bait and prey) is detected via in vivo reconstitution of a transcriptional activator that turns on expression of a reporter gene. While, in the one-hybrid system, detection is based on the interaction of a transcription factor (prey) with a bait DNA sequence upstream of a reporter gene. To ensure that DNA binding results in reporter-gene activation, cDNA expression libraries are used to produce hybrids between the prey and a strong transactivating domain. The advantage of cloning transcription factors or other DNA-binding proteins via one-hybrid screenings, compared to biochemical techniques, is that the procedure does not require specific optimization of in vitro conditions. This may circumvent possible difficulties, e.g., incorrect protein folding or lack of post-transcriptional modifications, that may occur in screenings for transcription factors via a prokaryotic expression system such as that used in the E. coli–based southwestern procedure.

Applications:

Identification of protein-DNA interactions
Screening for novel genes that bind to the cis-regulatory element
Elucidate mechanisms of action
Unravel pathways and molecular mechanism

Creative Biogene is one of the leading biotechnology companies which has extensive expertise and experience in providing Y1H cDNA library screening services for customers worldwide. Creative Biogene has established advanced technical platforms that can help you to study the interactions between the Y1H cDNA library and the specific DNA sequence of your interest. Creative Biogene has confident to provide you with the most affordable, high-quality Y1H cDNA library screening services to ensure your satisfaction in a timely and professional manner. If you are interested in our services, please do not hesitate to contact us. We are looking forward to your attractive projects.

References:

Sewell JA & Bass JIF. (2018). Options and considerations when using a yeast one-hybrid system. In Two-Hybrid Systems (pp. 119-130). Humana Press, New York, NY.
Ouwerkerk PB, & Meijer AH. (2011). Yeast one-hybrid screens for detection of transcription factor DNA interactions. In Plant Reverse Genetics (pp. 211-227). Humana Press, Totowa, NJ.
Ouwerkerk PB, & Meijer AH. (2001). Yeast one‐hybrid screening for DNA‐protein interactions. Current protocols in molecular biology, 55(1), 12-12.

* For research use only. Not intended for any clinical use.

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