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Over the past few decades, technologies surrounding DNA/RNA have grown in sophistication, allowing scientists to manipulate, edit, and even artificially synthesize these genetic modules. DNA/RNA extraction is often the first step in genomic research, clinical diagnosis or forensic analysis. During this manipulation, cellular structures are disrupted to release DNA/RNA, but this also releases other non-target cellular components. Although extraction protocols employ a series of washing and centrifugation steps to remove unwanted components, some unwanted impurities may still be present. These residual impurities may interfere with downstream applications such as PCR, sequencing, cloning, and in vitro transcription/translation, leading to erroneous conclusions and invalid results. Therefore, subsequent DNA/RNA cleanup steps are critical to achieve optimal yield and quality of genetic material.
DNA/RNA purification refers to the process of eliminating contaminants such as proteins, enzymes, free nucleotides, and residual reagents from DNA/RNA samples to ensure their purity and integrity for subsequent analysis or manipulation. Creative Biogene's various DNA/RNA cleanup kits provide a rapid method for the purification and cleanup RNA or DNA from different isolation methods or upstream applications. These kits can be used as an alternative to organic extraction and ethanol precipitation to purify various enzymatic reactions.
If you are unsure which assay and reagents are right for your application, please review our DNA/RNA Clean Up kit specific product pages, or contact a member of our team for a recommendation.
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