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Gel DNA recovery is a process of separating specific DNA fragments from agarose gel after gel electrophoresis. Agarose gel electrophoresis is a common technique for separating DNA fragments according to their size, which is widely used in molecular biology research. After electrophoresis, the DNA fragments are visualized by fluorescent dyes or ethidium bromide staining, and then gel DNA can be recovered to separate the specific fragments of interest for downstream applications.
Gel DNA recovery is necessary for the separation and purification of specific DNA fragments of interest from agarose gel after gel electrophoresis. This step is crucial in molecular biology research because it enables researchers to obtain high-quality DNA templates for downstream applications such as cloning, sequencing, PCR and genome editing. The recovery of gel DNA is carried out by various methods, including electroelution, crush-and-soak methods, as well as column-based purification. Among these methods, column-based purification using gel DNA recovery kit is the most popular and widely used because of its simplicity, high yield and reproducibility.
Creative Biogene provides various Gel DNA Recovery Kits, which can purify 100 bp to 20 kb DNA fragments from all levels of agarose gel with high recovery (>90%). These gel DNA recovery kits have an easy-to-follow protocol designed to maximize DNA purity and yield. These kits can be used for various DNA fragment sizes, all of which provide high-quality purified DNA for downstream applications. Purified DNA can be used for various downstream applications, such as ligation, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.
| Cat.No. | Product Name | Price |
|---|---|---|
| NKD-T002 | Gel Extraction 96 Kit | Inquiry |
| NKD-T005 | Gel/PCR DNA Kit | Inquiry |
| NKD-T006 | Gel Extraction Kit | Inquiry |
| NKD-T007 | Convenient Gel Kit | Inquiry |
| NKD-T008 | Acrylamide Gel DNA Kit | Inquiry |
| NKD-T009 | Gel MicroElute Kit | Inquiry |
| NKD-T029 | Gel Pure Quick Kit | Inquiry |
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