Fast qRT-PCR Surpermix Kit (Probe)

Name: Fast qRT-PCR Surpermix Kit (Probe)
SKU: QPQC-01
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QPQC-01

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Product Information

Cat. No.	QPQC-01
Description	Fast qRT-PCR Surpermix Kit (probe) is used in real-time quantitative PCR assays on fast cycling real-time thermal cycler platforms using fluorescent probe detection chemistry. The combination of fusion protein technology and optimized formulation helps perform fast duplex qPCR, while maintaining a broad dynamic range, reliability, and accurate quantification for robust gene expression analysis.
Sample Type	DNA
Report Gene	Probe based qPCR
Features	•Fast qPCR results: Fusion polymerase's stringent hot-start and rapid polymerization kinetics •Universal conditions: Fully compatible with your pre-existing cycling protocols and primer/probe conditions •Minimal PCR inhibition: highly tolerant polymerase and low PCR inhibition for better performance with fast cycling protocols •Distinct target detection: robust, simultaneous detection of different gene targets using probes under fast qPCR conditions
Applications	qPCR and two-step qRT-PCR in the hydrolysis probe detection format.
Selection Marker	DNA
Sensitivity	High
Method	Probe based
Storage	Store at -20°C and at dark condtion

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How does qPCR work?

A: qPCR relies on fluorescence from intercalating dyes or hydrolysis probes to measure DNA amplification after each thermal cycle. The most common dye-based method is SYBR Green and the most common probe-based method is TaqMan.

What is a probe in qPCR?

A: Probes are sequence-specific oligonucleotides with a reporter dye and quencher. The chemical reaction behind its mechanism is hydrolysis of the probe (cleavage of the reporter group from the quencher), resulting in the emission of a fluorescent signal.

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