Fast qRT-PCR Surpermix Kit (Probe)

Name: Fast qRT-PCR Surpermix Kit (Probe)
SKU: QPQC-01

For research use only. Not intended for any clinical use.

Cat.No.

QPQC-01

Description

Fast qRT-PCR Surpermix Kit (probe) is used in real-time quantitative PCR assays on fast cycling real-time thermal cycler platforms using fluorescent probe detection chemistry. The combination of fusion protein technology and optimized formulation helps perform fast duplex qPCR, while maintaining a broad dynamic range, reliability, and accurate quantification for robust gene expression analysis.

Applications

qPCR and two-step qRT-PCR in the hydrolysis probe detection format.

Features

•Fast qPCR results: Fusion polymerase's stringent hot-start and rapid polymerization kinetics
•Universal conditions: Fully compatible with your pre-existing cycling protocols and primer/probe conditions
•Minimal PCR inhibition: highly tolerant polymerase and low PCR inhibition for better performance with fast cycling protocols
•Distinct target detection: robust, simultaneous detection of different gene targets using probes under fast qPCR conditions

Method

Probe based

Report Gene

Probe based qPCR

Sample Type

DNA

Selection Marker

DNA

Sensitivity

High

Storage

Store at -20°C and at dark condtion

Publications

Q & A

Customer Reviews

Customer Q&As

How does qPCR work?

A: qPCR relies on fluorescence from intercalating dyes or hydrolysis probes to measure DNA amplification after each thermal cycle. The most common dye-based method is SYBR Green and the most common probe-based method is TaqMan.

What is a probe in qPCR?

A: Probes are sequence-specific oligonucleotides with a reporter dye and quencher. The chemical reaction behind its mechanism is hydrolysis of the probe (cleavage of the reporter group from the quencher), resulting in the emission of a fluorescent signal.

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