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QPHC-01

qPCR ToughMix Kit with ROX (probe)

For research use only. Not intended for any clinical use.

Cat. No. :   QPHC-01

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Cat. No. QPHC-01
Description qPCR ToughMix Kit with ROX (probe) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX. This kit contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, in the hydrolysis probe detection format.
Sample Type DNA
Report Gene Exclude primers or probe with ROX
Features •Increase qPCR sensitivity and specificity
•Produce lower cycle threshold (Ct) values
•Use the master mix with any probe-based assay
•Amplify and detect a broad range of DNA or cDNA targets
Applications qPCR and two-step qRT-PCR in the hydrolysis probe detection format.
Selection Marker DNA
Sensitivity High
Method Probe based
Note The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Storage Store at -20°C and at dark condtion
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What is the Rox passive reference in PCR?

A: Normalizing qPCR data using ROX reduces the effects of well-to-well variations such as uneven illumination, slight changes in optics, and differences in the amount of condensation.

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