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qPCR ToughMix Kit with ROX (probe)

For research use only. Not intended for any clinical use.
Cat.No.
QPHC-01
Description
qPCR ToughMix Kit with ROX (probe) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX. This kit contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, in the hydrolysis probe detection format.
Applications
qPCR and two-step qRT-PCR in the hydrolysis probe detection format.
Features
•Increase qPCR sensitivity and specificity
•Produce lower cycle threshold (Ct) values
•Use the master mix with any probe-based assay
•Amplify and detect a broad range of DNA or cDNA targets
Method
Probe based
Note
The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Report Gene
Exclude primers or probe with ROX
Sample Type
DNA
Selection Marker
DNA
Sensitivity
High
Storage
Store at -20°C and at dark condtion

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Customer Q&As
What is the Rox passive reference in PCR?

A: Normalizing qPCR data using ROX reduces the effects of well-to-well variations such as uneven illumination, slight changes in optics, and differences in the amount of condensation.

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