Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : QPHC-01
| Cat. No. | QPHC-01 |
| Description | qPCR ToughMix Kit with ROX (probe) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX. This kit contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, in the hydrolysis probe detection format. |
| Sample Type | DNA |
| Report Gene | Exclude primers or probe with ROX |
| Features |
•Increase qPCR sensitivity and specificity •Produce lower cycle threshold (Ct) values •Use the master mix with any probe-based assay •Amplify and detect a broad range of DNA or cDNA targets |
| Applications | qPCR and two-step qRT-PCR in the hydrolysis probe detection format. |
| Selection Marker | DNA |
| Sensitivity | High |
| Method | Probe based |
| Note | The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option. |
| Storage | Store at -20°C and at dark condtion |
A: Normalizing qPCR data using ROX reduces the effects of well-to-well variations such as uneven illumination, slight changes in optics, and differences in the amount of condensation.
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