qPCR Master Mix Kit with ROX (Probe)

Name: qPCR Master Mix Kit with ROX (Probe)
SKU: QPQC-02

For research use only. Not intended for any clinical use.

Cat.No.

QPQC-02

Description

qPCR Master Mix Kit with ROX (Probe) is supplied as a formulation for use in real-time quantitative PCR reactions (qPCR) with fluorescent probes requiring normalization with ROX. Simply add DNA template, primers, probe(s) and water and the reactions are ready for cycling. The master mix is formulated for use with fluorescent probes such as TaqMan Probes, Molecular Beacons, and others.

Applications

qPCR and two-step qRT-PCR in the hydrolysis probe detection format.

Features

•Convenient
•Multiple Platform Compatibility
•Novel Hot Start Technology
•Higher Specificity, Sensitivity and Broad Dynamic Range

Method

Probe based

Note

The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.

Report Gene

Probe based qPCR with ROX

Sample Type

DNA

Selection Marker

DNA

Sensitivity

High

Storage

Store at -20°C and at dark condtion

Publications

Q & A

Customer Reviews

Customer Q&As

What is the best amplicon length for qPCR?

A: Typical qPCR amplicon lengths are 70-200 bp, and we recommend designing your targets within this range.

Can probe-based qPCR detect multiple target sequences simultaneously?

A: Yes. Multiple probes with different fluorescent labels can be designed and used simultaneously in the same qPCR reaction. This enables simultaneous detection and quantification of multiple target sequences in a single run, saving time and resources.

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