qPCR Master Mix Kit with ROX (Probe)

Name: qPCR Master Mix Kit with ROX (Probe)
SKU: QPQC-02
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QPQC-02

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Product Information

Cat. No.	QPQC-02
Description	qPCR Master Mix Kit with ROX (Probe) is supplied as a formulation for use in real-time quantitative PCR reactions (qPCR) with fluorescent probes requiring normalization with ROX. Simply add DNA template, primers, probe(s) and water and the reactions are ready for cycling. The master mix is formulated for use with fluorescent probes such as TaqMan Probes, Molecular Beacons, and others.
Sample Type	DNA
Report Gene	Probe based qPCR with ROX
Features	•Convenient •Multiple Platform Compatibility •Novel Hot Start Technology •Higher Specificity, Sensitivity and Broad Dynamic Range
Applications	qPCR and two-step qRT-PCR in the hydrolysis probe detection format.
Selection Marker	DNA
Sensitivity	High
Method	Probe based
Note	The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Storage	Store at -20°C and at dark condtion

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What is the best amplicon length for qPCR?

A: Typical qPCR amplicon lengths are 70-200 bp, and we recommend designing your targets within this range.

Can probe-based qPCR detect multiple target sequences simultaneously?

A: Yes. Multiple probes with different fluorescent labels can be designed and used simultaneously in the same qPCR reaction. This enables simultaneous detection and quantification of multiple target sequences in a single run, saving time and resources.

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