qPCR ToughMix Kit (probe)

Name: qPCR ToughMix Kit (probe)
SKU: QPHC-02

For research use only. Not intended for any clinical use.

Cat.No.

QPHC-02

Description

qPCR ToughMix Kit (probe) is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. This kit contains all required reaction components, except primers, probe(s), and DNA template.

Applications

qPCR and two-step qRT-PCR in the hydrolysis probe detection format.

Features

•Increase qPCR sensitivity and specificity
•Produce lower cycle threshold (Ct) values
•Use the master mix with any probe-based assay
•Amplify and detect a broad range of DNA or cDNA targets

Method

Probe based

Report Gene

Exclude primers or probe

Sample Type

DNA

Selection Marker

DNA

Sensitivity

High

Storage

Store at -20°C and at dark condtion.

Publications

Q & A

Customer Reviews

Customer Q&As

What is the principle of probe in PCR?

A: The probe binds to a specific location on the target DNA sequence and fluoresces when cleaved by Taq polymerase during amplification. The amount of fluorescence produced is proportional to the amount of target DNA in the sample, allowing real-time quantification of DNA.

What is the advantage of probe-based qPCR?

A: The inclusion of a probe in the qPCR reaction can enhance the detection sensitivity compared to other qPCR methods. The probe specifically binds to the amplification product during the extension phase, generating a strong fluorescence signal only when the target sequence is present.

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